Authors
Nowroji Kavitha,Yeng Chen,Jagat R. Kanwar,Sreenivasan Sasidharan
Abstract
Phaleria macrocarpa
(Boerl.) is a well-known medicinal plant and have been extensively used
as traditional medicine for ages in treatment of various diseases.
The
purpose of this study was to determine the in situ cytotoxicity effect P. macrocarpa
fruit ethyl acetate fraction (PMEAF) by using various conventional and
modern microscopy techniques.
The cytotoxicity of PMEAF treated
MDA-MB-231 cells was determined through the
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
cytotoxicity assay and CyQuant Cell Proliferation Assay after 24 h of
treatment.
Both results were indicated that the PMEAF is a potential
anticancer agent with the average IC50 values of 18.10 μg/mL
by inhibiting the MDA-MB-231 cell proliferation.
Various conventional
and modern microscopy techniques such as light microscopy, holographic
microscopy, transmission (TEM) and scanning (SEM) electron microscope
were used for the observation of morphological changes in PMEAF treated
MDA-MB-231cells for 24 h.
The characteristic of apoptotic cell death
includes cell shrinkage, membrane blebs, chromatin condensation and the
formation of apoptotic bodies were observed.
PMEAF might be the best
candidate for developing more potent anticancer drugs or
chemo-preventive supplements.
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