En icke frisläppt forskningsartikel kommer snart publiceras i sin helhet.
Förmodligen via
American Chemical Society (ACS).
ACS beskriver sin gärning enligt följande:
Founded in 1876 and chartered by the U.S. Congress, we are the world’s
largest scientific society. Our mission is to advance the broader
chemistry enterprise and its practitioners for the benefit of Earth and
its people. Our vision is to improve people’s lives through the
transforming power of chemistry.
Det handlar om 11 forskare från Ungern och Japan med HoloMonitorfrälste Robert Horvath i spetsen.
Dessa forskare har gemensamt studerat ett område inom cancerforskningen som kommer mer och mer.
Nanopartiklar som bärare av cancerläkemedel.
Bloggen skrev om annan
aktuell forskning med nanopartiklar för några veckor sedan.
Hursom, nu har Robert m kollegor sammanställt sin studie betitlad :
Än så länge kan man bara läsa abstractet ur denna studie och där får vi förvisso ingen vägledning att man använt sig av PHI,s teknik.
Abstract
Functionalized nanoparticles (NPs) can penetrate into living cells and
vesicles, opening up an extensive range of novel directions. For
example, NPs are intensively employed in targeted drug delivery and
biomedical imaging. However, the real-time kinetics and dynamics of
NP–living cell interactions remained uncovered. In this study, we in
situ monitored the cellular uptake of gold NPs—functionalized with
positively charged alkaline thiol—into surface-adhered cancer cells, by
using a high-throughput label-free optical biosensor employing resonant
waveguide gratings. The characteristic kinetic curves upon NP exposure
of cell-coated biosensor surfaces were recorded and compared to the
kinetics of NP adsorption onto bare sensor surfaces. We demonstrated
that from the above kinetic information, one can conclude about the
interactions between the living cells and the NPs. Real-time biosensor
data suggested the cellular uptake of the functionalized NPs by an
active process. It was found that positively charged particles penetrate
into the cells more effectively than negatively charged control
particles, and the optimal size for the cellular uptake of the
positively charged particles is around 5 nm. These conclusions were
obtained in a cost-effective, fast, and high-throughput manner. The fate
of the NPs was further revealed by electron microscopy on NP-exposed
and subsequently fixed cells, well confirming the results obtained by
the biosensor. Moreover, an ultrastructural study demonstrated the
involvement of the endosomal–lysosomal system in the uptake of
functionalized NPs and suggested the type of the internalization
pathway.
MEN.
Eftersom Google är ett så användbart verktyg har bloggen lyckats komma in "bakvägen" till denna studie.
Nämligen via illustrationer i ett word-dokument tillhörande studien i fråga.
Där får vi bekräftat att HoloMonitor använts i forskningen.
Scrolla ner till sidan 4 i denna
länk. Där hittas följande information :
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Figure S5
B) 3D images of the cells,recorded by Holomonitor M4 holographic transmission microscope.
Väl bekomme.
Eder grävspade
the99
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