Forskningsrapport från Israel

17 israeliska forskare har fått sina studier kring elakartad hjärncancer hos barn publicerade i den vetenskapliga skriften International Journal of Cancer.

The long non‐coding RNA TP73‐AS1 promotes tumorigenicity of medulloblastoma cells

Abstract

Medulloblastoma is the most common malignant brain cancer in children.
Since previous studies have mainly focused on alterations in the coding genome, our understanding of the contribution of long non‐coding RNAs (lncRNAs) to medulloblastoma biology is just emerging. Using patient‐derived data, we show that the promoter of lncRNA TP73‐AS1 is hypomethylated and that the transcript is highly expressed in the SHH subgroup.
Furthermore, high expression of TP73‐AS1 is correlated with poor outcome in patients with TP53 wild type SHH tumors. Silencing TP73‐AS1 in medulloblastoma tumor cells induced apoptosis, while inhibited proliferation and migration were inhibited in culture.
In vivo, silencing TP73‐AS1 in medulloblastoma tumor cells resulted in reduced tumor growth, reduced proliferation of tumor cells, increased apoptosis leading to reduced survival of tumor bearing mice. Together, our study suggest that the lncRNA TP73‐AS1 is a prognostic marker and therapeutic target in medulloblastoma tumors and serves as a proof of concept that lncRNAs are important factors in the disease.

Denna forskningsrapport är låst som vanligt, men bakvägen via google får vi reda på användandet av HoloMonitor M4.

"A scratch was made with a tip and the cells were then placed in an incubator containing
holomonitor M4 device at 37°C and were …
The cells were tracked using holomonitor and their migration tracks were analyzed using M4 software … "


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