BioSpherix och PHI uppmärksammas av immunologisterna

Det amerikanska organet The American Association of Immunologists

är snälla och berättar för världens alla immunologister hur de ska undvika den beryktade " Edge Effect" när de labbar.Det gör de via deras tidskrift The Journal of Immunologi,men även via deras hemsida oxå.

Det handlar naturligtvis om postern bloggen har skrivit om vid ett flertal gånger tidigare.
Undertecknad förstod att det var något speciellt med denna nya "möjlighet" att kunna labba utan risk för temperatur och fuktskillnader. Såpass att det nu uppmärksammas av alltfler forskningsområden som sysslar med labbande. Vi hade American Association for Cancer Research i förra veckan och tidigare Toxikologisterna och nu alltså även Immunologiforskningen. Bloggen tror att vi kommer få se denna poster i flera sammanhang.

Controlled temp for cell plating eliminates room temp cell settling in 96-well plates, reducing Edge Effect and improving cell conditions

Alicia D. Henn, Shannon Darou, Stassa P.D. Henn, Allayna M. Frank, Kersti Alm and Randy Yerden

J Immunol May 1, 2019, 202 (1 Supplement) 131.14;

• Article
• Info & Metrics

Article Information

vol. 202 no. 1 Supplement 131.14

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Published By 

The American Association of Immunologists, Inc.

Print ISSN 

0022-1767

Online ISSN 

1550-6606

History 

• Published online July 3, 2019.

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Copyright & Usage 

Copyright © 2019 by The American Association of Immunologists, Inc.

Author Information

1. Alicia D. Henn1,
2. Shannon Darou1,
3. Stassa P.D. Henn1,
4. Allayna M. Frank1,
5. Kersti Alm2 and
6. Randy Yerden1

1. ¹BioSpherix
2. ²Phase Holographic Imaging, Sweden

Abstract

The “edge effect” has always limited the use of edge wells of widely-used 96-well plates, reduced useable assay space by 37%, increased materials, time, and costs for these assays. Methods reported to reduce edge effect include leaving the plates at room temperature (RT) for extended cell settling times (30–120 min). We previously reported that controlling conditions to 37° C during cell plating prevents thermal currents and non-random distribution of cells seen during cell settling in edge wells. Here we report studies with human A549 lung carcinoma cells, used for in vitro viral assays, and human bone marrow mesenchymal stromal cells (MSC). Our null hypothesis is that there would be no difference in cell density or viability between cells plated/settled at constant 37° C and extended cell settling times at RT conditions. We plated A549 or MSC under controlled conditions in modular cell handling and incubation chambers in the Xvivo System. We pre-incubated plates for 30, 60, 90, and 120 min at RT and compared these with cells handled at constant 37 degrees. We used the HoloMonitor M4 (PHI, Inc) to monitor cell settling and assessed cell density at 24 and 48 hrs later using crystal violet. We found that cell settling at RT resulted in delayed cell attachment, delayed time to first division, and different cell densities as compared with cell handling at 37° C. Cell agitation and thermal variation was still seen between center and edge wells during cell settling with medium warmed to 37 degrees before traditional cell plating at RT. We concluded that full-time control of 37° C during cell plating is necessary to prevent cell density and cell viability edge effect that occurs during routine handling of cells under RT conditions.

• Copyright © 2019 by The American Association of Immunologists, Inc.

Min kommentar
Onekligen är möjligheten att kunna labba utan "The Edge Effect" uppseväckande.
Vågar man tro att kombon från BioSpherix och PHI, Xvivo System optimized by HoloMonitor, kommer bli den nya standarden för världens alla labbare?
Kanske väl optimistiskt tänkt, men nog kommer kombon ligga högt på många labbares önskelista.
Med bra marknadsföring och tillgång till någon bjässes distributionsnät kanske ändå....?

                                           Mvh the99