Man gör bra PR för HoloMonitor.
Researcher Henriette Aksnes and Master student Tobias B. Beigl are pioneers exploring the possibilities of the new imaging system for their research on protein N-terminal acetylation in the Arnesen Lab.
The output of the HoloMonitor are phase-holographic images. These are different from regular phase contrast images as the microscope detects the surface of cells and produces a hologram.
These images therefore have three-dimensional information on the cell structure, which provides valuable research data. The 3D aspect is nicely visualized by colour-coding.
“The colourful 3D images and time-lapse videos of living cells are not only beautiful to look at”, says Tobias, “By analyzing the images we can quantify several different cellular parameters like cell size, proliferation or migration”.
Tobias is currently using the HoloMonitor for his master project where he wants to demonstrate some basal differences between two otherwise very similar cell types.
“The HoloMonitor is a very nice complement to the other microscopes, like the Leica SP8 STED and the Dragonfly spinning disk at the Molecular Imaging Center MIC, which we rather use for high-resolution imaging of fluorescently stained and fixed cells”, says Tobias.
Monitoring cells during their life in the culture dish reveals important characteristics of cell behavior and structure. Analyzing cell growth, cell division events or cell movement are just a few examples of the possible applications that can be easily evaluated with a software especially developed for the HoloMonitor system.
Monitoring cells during their life in the culture dish reveals important characteristics of cell behavior and structure. Analyzing cell growth, cell division events or cell movement are just a few examples of the possible applications that can be easily evaluated with a software especially developed for the HoloMonitor system.
Och avslutningsvis får vi i slutet på sidan information att man nu startat ett nytt forskningsprojekt där man med HoloMonitor ska studera proteinet i gen NAA80 i cancerceller.Huruvida dess karakteristik påverkar cancercellers förmåga att tränga in i omkringliggande vävnad såväl som genens inverkan på metastaser. Denna studie ser undertecknad fram mot att få läsa när den är klar.
These days, the HoloMonitor is running with cell culture samples prepared by master student Monica Hellesvik. Monica takes the NAA80 migration phenotype into a cancer perspective. She investigates whether these cells also have an increased invasive or metastatic capacity.
These days, the HoloMonitor is running with cell culture samples prepared by master student Monica Hellesvik. Monica takes the NAA80 migration phenotype into a cancer perspective. She investigates whether these cells also have an increased invasive or metastatic capacity.
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