Dessa forskare har använt sig av stamcellsforskning och lyckats identifiera ett ämne, Exosomes, med tidigare kända egenskaper att kunna kommunicera cell-till-cell,till att kunna applicera detta ämne till celler som påverkar människas motorik.Forskarna renade/koncentrerade signalämnet Exosomes och tillförde det till kända nervceller (SH-SY5Y-celler) som tillverkar dopamin vilka är nödvändiga för att styra människas motorik.När kroppen inte längre kan producera tillräcklig mängd dopamin leder det till försämrad motorik.Patienter som tidigt diagnostiserats med Parkinson (otillräcklig dopaminproduktion) har en sjunkande produktion av ämnet vilket konstant försämrar motoriken,den förvärras hela tiden.Forskare och läkare har inte kunnat få stopp på patients gradvisa försämring utan enbart i viss grad kunnat mildra symtomen.
Men nu har alltså dessa forskare kanske hittat lösningen på detta problem.Med att tillföra Exosomes till nervceller som gått in i apoptos (celldöd) kan forskarna visa på att celldöden stannar upp och så även förloppet i sjukdomens utveckling.
Hos Svenska Parkinson Förbundet kan man läsa mer om sjukdomen.
Hebei Medical University finns med på PHI`s sida Users.
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| The First Hospital Of Hebei Medical University is located in Shijiazhuang, Hebei, China and is part of the Hospitals Industry. The First Hospital Of Hebei Medical University has 2,119 total employees across all of its locations. There are 5 companies in the The First Hospital Of Hebei Medical University corporate family. |
Men till studien.
Exosomes derived from mesenchymal stem cells repair a Parkinson’s disease model by inducing autophagy
Abstract
Parkinson’s disease (PD) is a progressively debilitating neurodegenerative condition that leads to motor and cognitive dysfunction. At present, clinical treatment can only improve symptoms, but cannot effectively protect dopaminergic neurons. Several reports have demonstrated that human umbilical cord mesenchymal stem cells (hucMSCs) afford neuroprotection, while their application is limited because of their uncontrollable differentiation and other reasons. Stem cells communicate with cells through secreted exosomes (Exos), the present study aimed to explore whether Exos secreted by hucMSCs could function instead of hucMSCs. hucMSCs were successfully isolated and characterized, and shown to contribute to 6-hydroxydopamine (6-OHDA)-stimulated SH-SY5Y cell proliferation; hucMSC-derived Exos were also involved in this process. The Exos were purified and identified, and then labeled with PKH 26, it was found that the Exos could be efficiently taken up by SH-SY5Y cells after 12 h of incubation. Pretreatment with Exos promoted 6-OHDA-stimulated SH-SY5Y cells to proliferate and inhibited apoptosis by inducing autophagy. Furthermore, Exos reached the substantia nigra through the blood–brain barrier (BBB) in vivo, relieved apomorphine-induced asymmetric rotation, reduced substantia nigra dopaminergic neuron loss and apoptosis, and upregulated the level of dopamine in the striatum. These results demonstrate that hucMSCs-Exos have a treatment capability for PD and can traverse the BBB, indicating their potential for the effective treatment of PD.
Jag klipper som vanligt in enbart Holorelaterat.
Material and Methods
The proliferation of cells was measured using a HoloMonitor® time-lapse cytometer (BIO-SUN SCI&TECH, Beijing, China). Cells at passage 3 were seeded into 6-well plates at 1.3 × 105 cells per well. They were observed for 6 days, and the medium was replaced after 3 days. FCM was performed to detect marker expression using the following fluorescein isothiocyanate (FITC)-conjugated or phycoerythrin-conjugated antibodies: CD73, CD45, CD34, CD90, CD105, and HLA-DR (BD Biosciences®, Sparks, MD, USA).
Detta är forskningsrapport nr 150 PHI nu kan stoltsera med.
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