Angelägen forskningsrapport

11 forskare från bla Northeastern University och Children´s Hospital of Philadelphia har fått sina studier om neutrophil extracellular traps (NETs) godkända och publicerade.Vad dessa NETs har för funktion och fungerar är alldeles för komplicerat för undertecknad att förklara,så därför överlåter jag detta till en forskare som vet vad han pratar om.På denna youtubefilm får ni all info ni behöver.

Men till forskningsrapporten då :

Monoclonal antibody 2C5 specifically targets neutrophil extracellular traps

Published online: 15 Dec 2020

ABSTRACT

Neutrophils can release DNA and granular cytoplasmic proteins that form smooth filaments of stacked nucleosomes (NS). These structures, called neutrophil extracellular traps (NETs), are involved in multiple pathological processes, and NET formation and removal are clinically significant. The monoclonal antibody 2C5 has strong specificity toward intact NS but not to individual NS components, indicating that 2C5 could potentially target NS in NETs. In this study, NETs were generated in vitro using neutrophils and HL-60 cells differentiated into granulocyte-like cells. The specificity of 2C5 toward NETs was evaluated by ELISA, which showed that it binds to NETs with the specificity similar to that for purified nucleohistone substrate. Immunofluorescence showed that 2C5 stains NETs in both static and perfused microfluidic cell cultures, even after NET compaction. Modification of liposomes with 2C5 dramatically enhanced liposome association with NETs. Our results suggest that 2C5 could be used to identify and visualize NETs and serve as a ligand for NET-targeted diagnostics and therapies.

Materials and Methods (urval)

Phase holographic imaging

Phase holographic imaging is a laser-based methodology that captures time-lapse images of unlabeled cells and allows for observation and quantification of differences in cell morphology based on holographic reconstructions of the cells’ refractive index. ^51,53 For holographic microscopy, cells were seeded on glass-bottom, 24-well plates and incubated in the presence or absence of ATRA and DMSO. The Holomonitor M4 (Phase Holographic Imaging, Lund, Sweden) was used to collect images every 10 minutes over 5 days from each well (3 regions/well) using a 20x objective lens. The data collected were analyzed using Hstudio analysis software (Phase Holographic Imaging) to characterize the morphological features of cells in the differentiation process.

 

 

(B)Images of HL60 cells exposed to DMSO or ATRA. Phase holographic images show differences in size and morphology of cells differentiated in the presence of ATRA or DMSO for 3 days. Cell volume was analyzed with Hstudio analysis software (Phase Holographic Imaging) at 4h and 72h after incubation with the differentiation agents. The graphs for each group and time-point are presented below the images, showing changes in cell size after differentiation. Scale bar = 100 µm

Min kommentar

Studien ser ut att handla om kroppens immunförsvar vid en blodcancer / leukemi.Eftersom forskare från ett barnsjukhus är inblandade förmodar jag att det berör barn som drabbats av denna förb...e cancerform.

Det hedrar forskarna från PHI`s partner Northeastern University (Ed Luther,Livia Mendes,Vladimir Torchilin bla..) att de hjälper sina kollegor på barnsjukhuset i Philadelphia med sina kunskaper och även erfarenheter av HoloMonitor för att bringa mer kunskap i att bekämpa denna eländiga best. Hjärtat blöder lite extra när barn drabbas av cancer.

Måtte forskarna hitta nya och bättre vägar för att förinta detta (censur) monster.

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