Patent

 Skrev en kommentar på börssnack till mitt inlägg jag la ut i morse.

- Ingen som listat ut att denna nya Glycofunktion har koppling till det nya patentet?

Ovanstående studie förbättringen berättar om accepterades 23 Mars.

Received: December 7, 2020 Accepted: March 23, 202. Publication Date:April 20, 2021

Och den 31 Mars söktes ett nytt Glycopatent. Et voilá

 

Sen kollade jag bloggen på ev kommentarer.Och visst fanns det 1.

Mr anonym grävare har lagt ut länk till ett patent PHI själva sökt.

Länk

 

Applicants

• PHASE HOLOGRAPHIC IMAGING PHI AB [SE]/[SE]

Inventors

• EGELBERG, Peter
• BERGKVIST, Jonas

Kan vara idag patentet blev sökbart på nätet.

 

 

Själva patenten ser ut att ha sökts för 1 år sen. Innehållet med beskrivning vibbar starkt om Fluo-koppling.Jag klipper in inledningen på beskrivningen.Läs hela texten här.

Field of the invention

The invention relates to a microplate for microscopy of an organoid and to a method for microscopy of an organoid.

Background of the invention

A common container for growing cells, such as in an organoid, is called a microplate. Microplates are flat plates, most often made of plastic, which have a plurality of wells arranged in a matrix on them. Each well may typically hold between several nanoliters and several milliliters of liquid and are used as small test tubes in which cells may be cultured. Typical microplates may have anywhere between 6 and 1536 wells arranged in a rectangular matrix. The wells are typically formed as cylinders in the microplate having an open top and a closed bottom such that they may hold a small amount of liquid.

When growing cells in a well of a microplate it is desirable to be able to monitor the cells, for instance to monitor the cell growth or the current number of cells. The microplate may thus be placed in a microscope such that the cells in the well are observed either from above or from below the well, most often in a direction along the depth extension of the well. For this reason, the microplates, or at least the portion of the microplate which is situated under a well, are made transparent such that the cells in a well can be monitored by a microscope.

Since most cells are transparent it may be difficult to monitor them using a regular optical microscope. In order to better observe the cells and cellular structures, the cells and cellular structures may be colored prior to being observed. Flowever, such colorings often result in the death of the cells which prevents phenomena of living cells from being observed.

An alternative technique is to attach fluorescent molecules to living cells. Then by directing an excitation beam of light with a suitable wavelength towards the cells the fluorescent molecules will be excited and send out colored light of their own which light may be observed in a microscope.

Typically, the excitation beam of light is a laser beam. An advantage of attaching fluorescent molecules is that it allows for the monitoring of just a portion of the cells by only illuminating that portion with the excitation beam of light.

A problem with conventional microplates is that they only allow vertical illumination, and thus excitation, of the fluorescent molecules. Thus it will only be possible to observe portions of the cells which extend in a vertical direction.

It is not possible to illuminate, and thus excite the fluorescent molecules of, portions of the cells which extend in a horizontal direction, that is a direction which is perpendicular to the imaging axis. This as the microplate itself is in the way of the particular well of interest.

Thus, improvements to microplates which allows for horizontal illumination of cells in a well is desired.

Min kommentar

Sannolikheten för att detta patent är en förberedelse inför Holo/Fluo-kombin är mer än hög.Varför söker man annars patent för möjlighet att studera fluorescenta (stavning?) objekt,celler i befintlig utrustning?Håller tesen är vi inte alltför långt ifrån en launchning av denna efterlängtade kombo.Lägg till Glycoinformationen.Vad får vi då?

                                                 Mvh the superexcited 99