Kinesiska forskare prisar Digital Holografisk Mikroskopi (DHM) i en studie publicerad i en edition till Nature. Det som den bästa tekniken att studera mannens spermier. Bloggen har tidigare uppmärksammat forskare som använt DHM just för fertilitetsforskning (När 2 blir 1),finns flera studier på bloggen om detta ämne.Använd sökrutan för att hitta dessa.
A Preliminary Study on the Evaluation of Human Sperm Head Morphology with a Domestic Digital Holographic Image System
Abstract
The head of sperm was imaged with domestic digital holographic microscopy (DHM), and then the quantitative three-dimensional size information of normal sperm and teratozoospermic sperm was compared and analyzed. DHM sperm imaging and repeated quantitative evaluation were used to determine the morphology of the sperm head in two patients with teratozoospermia and four volunteers with normal semen parameters. Sixty and 139 sperm of teratozoospermia patients and normal people were photographed by digital hologram, respectively. The differences in head height and width were compared and statistically analyzed. The sperm head height of the teratozoospermia group was 3.06 ± 1.66 μm, which was significantly lower than that of the normal sperm group (4.54 ± 1.60 μm, p < 0.01), but there was no significant difference in the head width between the two groups. Compared with the traditional two-dimensional optical microscope observation method, the DHM system can provide three-dimensional quantitative information for the sperm head and thus may help in the comprehensive clinical evaluation of the sperm head structure.
Klipp från studien. (understrykningar är mina egna)
DHM is an epochal high-tech product. It is a comprehensive technique involving information optics, holography, optoelectronics and digital image processing technology. At present, research in Europe, the United States, Japan and Singapore is very active. Commoditized companies include Sweden HoloMonitor, Switzerland Lyumcee Tec, Belgium Ovizio and so on. The price of imported products is usually approximately 2 million RMB. FM-DHM 500 is the first commercial DHM in China. It can realize the quantitative information reconstruction of the three-dimensional structure of the sample surface with a single exposure. It has the advantages of laser scanning confocal microscopy for 3D imaging without scanning the sample. It can realize dynamic three-dimensional topography measurements with micro- and nano-precision. Quantitative analysis, automatic identification of cells and microorganisms, and dynamic tracking analysis of multiple cells can be performed. The acquisition and reconstruction speed is very fast, which allows the image to be viewed in real time. Biological samples can be viewed directly without cell marking and staining.
DHM has the advantages of being noncontact, nondestructive, unmarked and high resolution, because it can obtain the amplitude and phase information of the object in the light field (Kolman and Chmelík 2010). Therefore, it is very suitable for microscopic observation and quantitative analysis of biological samples, especially living tissues and cells, and has become a hot spot in the application research of digital holography (Sohn et al. 2011). The experimental results of HeLa cells show that DHM can dynamically obtain high-resolution microimages of cell phase differences, which can be used to analyze the growth, change and movement of cells (Belashov et al. 2020). By further optimizing experimental equipment and reconstruction algorithms, DHM will be widely used for dynamic observation of living tissues and cells, thus promoting the understanding of biological processes and the development of life science research (Lee et al. 2011).
Conclusion
Currently, computer-assisted sperm analysis systems used in clinics and researches still use a 2D view of sperm movement and morphology. In July 2020, the Science Advances journal reported that using 3D microscopy and digital technology, an optical illusion of sperm tail movement was discovered. The tail of sperm appears to move in a symmetrical way, but in fact, the tail moves only sideways, which seems to mean that the sperm can swim only forward in a circle (Gadêlha et al. 2020). This will revolutionize the understanding of sperm motility and its impact on natural fertilization, laying the foundation for the identification of unhealthy motility sperm and the development of diagnostic tools.
Similarly, the three-dimensional evaluation of sperm head height by DHM has potential value in clinical practice for assessing the complete structure and composition of sperm heads; in particular, the relationship between sperm head structure and sperm fertilization ability and embryo development is worth further study. In addition, it is necessary to establish a standardized operation process of DHM to facilitate clinical standardization.
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