Årets första forskningsrapport (Nature)

2023 börjar med en rivstart beträffande forskningsrapporter. I en edition till Nature får 4 kinesiska forskare sina studier om maligna (pot dödliga) tumörer publicerade. Studien publicerades igår 5/1 2023 i organet BioMedCentral (BMC).

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Ämnet i studien är hur ett kroppseget protein, FGF23, påverkar cancercellers förmåga att växa i antal och bilda tumörer.Det är sen tidigare känt att proteinet ifråga medverkar till att utveckla prostatacancer,dock har forskare på senare tid identifierat proteinet i andra cancerformer. De kinesiska forskarna har gett sig i kast med att se om det finns en koppling mellan proteinet och en jobbig cancer som nästan alltid enbart drabbar barn och ungdomar. Nämligen OsteoSarcoma (OS) som är en bencancer ofta spridd till skelettet. Resultatet av deras forskning är hoppingivande för de barn som drabbas av denna cancer,därav är deras studier så viktiga och kommer förhoppningsvis leda till fler studier med lösning i hur bota denna eländiga cancer. Men till studien betitlad :

FGF23 promotes proliferation, migration and invasion by regulating miR-340-5p in osteosarcoma

• Published: 05 January 2023
  

 

Osteosarcoma (OS) is the most common primary malignant bone tumor, mainly occurring in children and adolescents. It occurs frequently in tubular bones of limbs, resulting in limited joint movement, fractures, and even pulmonary metastasis, with high incidence and poor prognosis. Surgical tumor resection, systemic chemotherapy, and targeted radiotherapy are currently the standard treatment strategies for osteosarcoma [1], and the prognosis and survival rate of patients with non-metastatic osteosarcoma have been significantly improved, but the 5-year survival rate of patients remains very low due to the high aggressive and rapid metastatic nature of OS [2]. Therefore, it is essential to strengthen the study of the molecular mechanism of osteosarcoma.

Abstract

Background

Increasing evidences have been indicated that FGF23 is associated with the biological behavior of malignant tumors, but its role in osteosarcoma and the specific mechanism need to be elucidated. The purpose of this study is to investigate the effects of FGF23 on the proliferation, migration and invasion of osteosarcoma cells, and the possible molecular mechanisms.

Methods

Western blot was used to detect differences in FGF23 expression in osteosarcoma cells MG-63 and U2-OS and osteoblasts hFOB1.19. FGF23-overexpressing adenoviruses and FGF-silencing plasmids were transfected into osteosarcoma cells, and transfection efficiency was verified using Western blot. MTT and colony formation assays were performed to detect osteosarcoma cell proliferation. Cell cycle was measured by flow cytometry. Scratch assay, holographic imaging cell analyzer Holomonitor ® M4 and transwell were applied to detect cell migration and invasion. Dual-luciferase reporter assay was performed to validate the interaction between FGF23 and miR-340-5p. Changes in miR-340-5p mRNA levels were measured by QRT-PCR.

Results

FGF23 is highly expressed in osteosarcoma cells compared to hFOB1.19. Overexpression of FGF23 significantly promoted the proliferation, migration and invasion of MG-63 and U2-OS cells. MiR-340-5p is a target of FGF23. Transfection of miR-340-5p mimics reversed the promoting effects of FGF23 on proliferation, migration and invasion of MG-63 and U2-OS cells.

Conclusion

FGF23 promotes osteosarcoma cell proliferation, migration and invasion by targeting miR-340-5p gene expression.

Som synes redan i ingressen redogör man vilket instrument som varit av betydelse för studiens resultat.

Materials and Methods

Holomonitor ® M4

Motion trajectories of U2-OS and MG-63 cells were tracked and imaged using a Holomonitor ® M4 microscope [15]. Open the microscope component of the Holomonitor ® M4 system and place it in the CO₂ incubator overnight to improve the stability of each parameter. Cells were seeded in a matched six-well plate at a density of 2 × 10⁵ cells/well, processed according to predetermined groups, and placed in the instrument. After testing the instrument and device, observe continuously for 24 h, with each time at an interval of 10 min. 24 h later, M4 Studio tracking software 2.6.2 was used to analyze the data.

FGF23 promotes the migration and invasion of osteosarcoma cells

The effect of interfering with FGF23 expression on the migration of OS cells was studied by scratch assay (Fig. 3A). The results showed that the cell migration ability of ad-FGF23 group was significantly higher than that of NC group. Moreover, individual cells were followed up by a holographic cell imaging analyzer, and the migration distance of cells in the ad-FGF23 group was found to be significantly increased compared with the control group (Fig. 3B).

FGF23 promotes osteosarcoma cell migration and invasion. A Scratch assay was used to detect the effect of interfering with FGF23 expression on osteosarcoma cell migration. B Holomonitor ® M4 was used to track the trajectory of single cell motility.

 

 

FGF23 promotes osteosarcoma cell migration and invasion by inhibiting miR-340-5p. A, B Scratch assay and holographic cell imaging showed that miR-340-5p inhibitor could reverse the decreased migration ability caused by FGF23 silencing.

Conclusion

FGF23 is highly expressed in osteosarcoma cells and can promote the ability of osteosarcoma cells to proliferate and migrate. MiR-340-5p is identified as a direct target gene of FGF23. FGF23 can target miR-340-5p to regulate the development and progression of osteosarcoma cells. The results of this study suggest that FGF23 may be an effective molecular target for the clinical treatment of osteosarcoma.

Min kommentar

Forskarna har mest troligt hittat den kommande lösningen för att ha ihjäl denna cancerform.Att sikta in sig på att neutralisera proteinet FGF23 i så tidigt stadie som möjligt.Det ger förhoppningsvis större chans till överlevnad och bra mycket större chans till att få tumören att stanna upp och inte sprida sig vidare in i kroppen.I användandet av HoloMonitor tror jag mig förstå att forskarna använt sig av Single Cell Analysis (SCA). 

Den teknik som 2020 identifierades som en av de viktigaste i framtida cancerbekämpning.

The Single-Cell Analysis Revolution Lund, January 2, 2020

Chefen för statliga NIH, Francis Collins, sa i en interju vid den tiden följande om de 10 viktigaste kommande teknikerna inom sjukvårdsområdet (notera vilken plats Francis åsätter SCA) :

 

Emerging technologies look set to transform the healthcare industry. In this year's Global Innovation Index, Francis Collins, Director of the National Institutes of Health (NIH), explores some of the innovations that could have the biggest impact in the next decade. Here's a summary of those findings from the report. 

Every day, medical innovations lengthen and improve lives across the globe. Over the course of the next decade, as twenty-first century technologies combine and accelerate, healthcare is set for a revolution.
These trends are leading to breakthroughs across a range of medical frontiers. For this year’s GII, the NIH identified 10 of the cutting-edge emerging technologies most likely to revolutionize healthcare over the next decade.

Here’s a closer look at the technologies that made the list:

1. Single cell analysis
Likely to be one of the first of the 10 breakthroughs to come to fruition, single-cell analysis will allow scientists to study individual cells in their normal environment for the first time. The ability to determine which genes are turned on or off in individual cells, and to decode how immune cells attack healthy tissue, will transform how we approach autoimmune diseases and how we combat the deadly process of cancer metastasis. "

Nu 3 år senare ser vi hur forskarna börjar anamma tekniken och listar ut hur använda HoloMonitor på effektivt sätt.Måtte fler forskare göra samma upptäckter.Cancer är en best som kräver de bästa instrumenten för att överlistas. För övrigt kan nämnas att studien uppmärksammats av och lagts upp hos

 

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Ps. I helgen kommer ett inlägg jag tror alla phi,are vill ta del av. Ds

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