Oscar mejlade över en ny studie där resultaten kommer ha stor betydelse för framtida bröstcancerforskning samt vid framtagning av nya läkemedel mot denna best.
Comparative Morphological Analysis of MCF10A and MCF7 Cells Using Holographic Time-lapse Microscopy
Studien har såpass bra impact att statliga NIH var snabba att lägga upp den i sitt bibliotek.
Comparative Morphological Analysis of MCF10A and MCF7 Cells Using Holographic Time-lapse Microscopy
Först hos ursprungsstället studien publicerades på, ANTICANCER RESEARCH.
Sen hos NIH.
Ni förstår säkert varför när ni jämför sidorna.(om inte kolla mina fetningar)
Background/aim: Breast cancer, one of the most prevalent cancers globally, is marked by its cellular heterogeneity. A key aspect of breast cancer research is understanding the distinct morphological features of cancerous and non-cancerous cells, which could serve as potential targets for novel therapeutic interventions. In this light, our study aimed to comprehensively analyze the morphological features of the MCF10A and MCF7 cell lines, representing normal breast and breast cancer cells, respectively. The ultimate objective was to identify the most significant features that differentiate these cell lines.
The primary features assessed in our study included texture clustershade, area (μm2), eccentricity, irregularity, phaseshift sum, optical volume (μm3), shape convexity, and Hull convexity.
Conclusion: The identified morphological differences between MCF10A and MCF7 cells offer valuable insights into the characteristics distinguishing normal breast cells from breast cancer cells. These findings not only contribute to our understanding of the morphological variability in breast cancer but also underscore the potential utility of these differences in future cancer diagnostics and treatment strategies.
Materials and Methods (urval)
MCF10A cells were seeded in the 24-well plates 24 h before cells were visualized under a holomonitor. MCF10A cells were maintained in MEBM Basal Medium supplemented with BPE, hEGF, Insulin, hydrocortisone, GA-1000 (Lonza, Basel, Switzerland). MCF7 cells were maintained in DMEM medium (Gibco, New York City, NY, USA), 10% FBS (HyClone, Logan, UT, USA), 5% Penicillin/Streptomycin, Insulin (1 mg/ml, Gibco) at 37°C with 5% CO2.
The digital holographic monitor investigations were carried out with a HoloMonitor M4, produced by Phase Holographic Imaging (PHI, Lund, Sweden), which was placed inside the mammalian cell culture incubator at 37°C with a 5% CO2 environment. Time-lapse phase imaging, alongside image processing, segmentation, and analysis were performed via the Hstudio software package provided by PHI. The proliferation of cells was evaluated by imaging every 15 min following 24 h after plating. In each well, the images were segmented, identified and cells were counted using the Cell Count module in HStudio software. For the tracking assays mentioned standard culture conditions were followed and cells were plated in 24-well plates (Sarstedt, Hildesheim, Germany).
In this study, we conducted a comprehensive analysis of morphological feature changes between MCF10A, a normal breast cell line, and MCF7, a breast cancer cell line (Figure 1). In our analysis of the breast cancer dataset, we aimed to identify the morphological features that most significantly differentiate between MCF10A and MCF7 cell lines. To achieve this, we employed a feature selection technique based on mutual information, followed by t-tests to assess the statistical significance of the differences observed. We also generated boxplots, violin plots, and density plots for each of the top 10 features to visually inspect the distributions of these features for the two cell lines.
 |
| Figure 1. |
Digital Holographic images of the (A). MCF10A and (B). MCF7 pseudo colored using HStudio.
Bilden ovan visar alltså hur HoloMonitor särskiljer friska celler (vänster bild) från cancerceller.
 |
| Figure 2. Box plots and violin plots of the texture clustershade, area, eccentricity, irregularity, optical volume, and phaseshift sum. Images were taken with the PHI digital phase imaging microscope (PHI, Lund, Sweden). Images were processed and analyzed using Hstudio. A, B: Violin and box plots of texture clustershade; C, D: Violin and box plots of area; E, F: Violin and box plots of eccentricity; G, H: Violin and box plots of irregularity; I, J: Violin and box plots of Otical volume; K, L: phaseshift sum. |
 |
| Figure 3. Box plots and violin plots of the shape and hull convexity. Images were taken with the PHI digital phase imaging microscope (PHI, Lund, Sweden). Images were processed and analyzed using Hstudio. A, B: Violin and box plots of shape convexity; C, D: Violin and box plots of Hull convexity. |
Jag får återigen upprepa mig likt en papegoja : HoloMonitor är en cancerforskares kanske bästa vän.
Som service till alla ev HoloMonitornyfikna forskare : HoloMonitor Demo
Inga kommentarer:
Skicka en kommentar