2 forskningsrapporter varav 1 från Springerkoncernen (Nature) och en blänkare i en sajt för labbpersonal.
I denna Springer publikation är det forskare från PHI`s partner Northeastern University, Boston som studerat olika läkemedels effektivitet att bekämpa cancertumörer.
Abstract
Combination therapy with small interfering RNA (siRNA) and chemotherapeutic drug is proven to be effective in downregulating cancer resistance proteins, such as P-glycoprotein (P-gp). These proteins are involved in multidrug resistance (MDR) of tumors. A targeted formulation capable of delivering siRNA and chemotherapeutic drug will not only downregulate P-gp but also increase the concentration of the chemotherapeutic drug at the site of tumor thereby increasing the therapeutic effect and lowering the systemic exposure. In this study, monoclonal antibody 2C5-modified dendrimer-based micelles were used to co-deliver siRNA and doxorubicin (DOX) to the tumor site in both male and female xenograft mouse model. The nucleosome-specific 2C5 antibody recognizes the cancer cells via the cell-surface bound nucleosomes. The ability of ability of the 2C5-modified formulation to affect the metastasis of highly aggressive triple negative breast cancer cell migration in (MDA-MB-231) was assessed by a wound healing.
Wound healing assay
MDA-MB-231 cell suspensions grown in T-75 flasks to 85% confluence were harvested. Cell suspension of 70 µL was pipetted into the two wells of each insert (15,000 cells). Inserts (one per well) were placed in a sterile 24 well dish. Cells were incubated for 24 h at 37 °C to allow sedimentation and attachment. The cells were treated with formulation independently at a final concentration of 2.5 µg/mL, The inserts were removed with sterile tweezers and 1 mL medium was added per well to remove unattached cells. One additional mL of medium was added to the wells before imaging. The morphology of the cells was recorded using a Holomonitor. The wound was imaged holographically over 48 h (at 10-min intervals) to secure quantitative data on gap widths and the percent cell-free areas following the drug treatments. Inserts were introduced inside wells followed by cell seeding, and once a confluent monolayer was produced, the inserts were removed. Thus, the removal of the blockade caused by the insert, resulted in a uniform cell-free zone, into which cells could migrate. The process of migration was followed by a live-cell imaging technique such as holographic monitoring or using regular microscopy.
Här är det tjeckiska forskare som studerat cancerformen Melanoma. Som oftast förekommer i huden eller i sällsynta fall även vid ögon,magen,munhålan och genitalierna,
Introduction
Melanoma is a cancer stemming from malignant melanin-producing cells – melanocytes. It primarily occurs in the skin but may also originate in the eyes, the gastrointestinal tract, or oral, genital, and nasal mucous membranes. The incidence of this cancer has expanded in developed countries, such as the US and the UK. Melanoma accounts for 80% of skin cancer deaths, and it has been estimated that 57,000 have died of melanoma in 2020. It is at the forefront of cancer diagnosis in the developed world, and projections point towards an increase in incidence in the coming decades. For patients diagnosed with stage IV of the disease, the survival rate is dismal, though overall mortality rates have decreased due to advances in targeted- and immuno-therapies.
Materials and Methods (urval)
2.12.2 Proliferation
The kinetic measurement of cell growth was performed using holographic microscopy. B16F10 cells were seeded in 96-well plates (Lumox multiwell, Sarstedt; 5,000 cells per well). The next day, ESP fractions (final concentration 50 µg/ml) in complete medium (DMEM supplemented with 10% FBS and antibiotics) were prepared from stock solutions and added to the cells (170 µL per well). A complete medium without ESP was used as a positive control, and two concentrations of Vincristine (VCR) were used as negative controls. The wells were covered with HoloLid (PHI AB). Cell count measurement was performed on a HoloMonitor microscope (PHI AB) using App Suite Imaging Software (PHI AB). Each time point in each condition represents the mean of cell counts of four positions. Two independent experiments were performed.
Och slutligen. HoloMonitor har nu lagts till i sajten Lab Bulletins förteckning över instrument labbfolk kan få info om och även beställa.
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Länk
HoloMonitor M4 + Fluorescence = HoloMonitor M4FL
The holomonitor fluorescence module is a new add-on compatible with the HoloMonitor M4 system. With the M4FL add-on, you can tailor HoloMonitor Live Cell Assays to your needs and add fluorescence to your HoloMonitor system to best fit your research application.
What is the Holomonitor?
The HoloMonitor live cell imaging system is your comprehensive single-cell level imaging solution, seamlessly fitting within a standard CO₂ incubator.
The HoloMonitor employs digital holographic microscopy to allow non-invasive visualization and quantification of living cells without compromising cell integrity.
An advantage of holographic microscopy is that the created quantitative phase images are focused when viewed, not when recorded. This makes the HoloMonitor ideal for long-term imaging by time-lapse microscopy,
The HoloMonitor M4FL is now available from Thistle Scientific.
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Som service till alla ev HoloMonitornyfikna forskare : HoloMonitor Demo
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