PHI toppar kanske inte populäritetslistan just nu,men HoloMonitor ligger iaf i topp hos de initierade.
Methods to Investigate Cell Migration
Abstract
Cell migration is of great relevance to researchers studying development, wound healing, the immune system and its responses and diseases associated with deregulated cell migration. The study of cell migration has been made possible by the development of a huge variety of experimental set-ups in 2D and 3D focussing on specific aspects of cell migration. In this chapter, some of such specific aspects of cell migration will be discussed and the assays available to investigate the particular features of cell migration. Examples of traditional cell migration assays and most recent developments ranging from wound healing assays to microfluidics studies will be given, followed by commonly used assays in a 3D setting and finally highly specialised intravital in vivo research. The advantages and disadvantages of such assays are highlighted.
Mer från Springer/Nature :
KHDRBS1 regulates the pentose phosphate pathway and malignancy of GBM through SNORD51-mediated polyadenylation of ZBED6 pre-mRNA
- Published:
Abstract
Glioblastoma is one of the most common and aggressive primary brain tumors.
The aberration of metabolism is the important character of GBM cells and is tightly related to the malignancy of GBM. We mainly verified the regulatory effects of KHDRBS1, SNORD51 and ZBED6 on pentose phosphate pathway and malignant biological behavior in glioblastoma cells, such as proliferation, migration and invasion. KHDRBS1 and SNORD51 were upregulated in GBM tissues and cells. But ZBED6 had opposite tendency in GBM tissues and cells. KHDRBS1 may improve the stability of SNORD51 by binding to SNORD51, thus elevating the expression of SNORD51. More importantly, SNORD51 can competitively bind to WDR33 with 3’UTR of ZBED6 pre-mRNA which can inhibit the 3’ end processing of ZBED6 pre-mRNA, thereby inhibiting the expression of ZBED6 mRNA. ZBED6 inhibited the transcription of G6PD by binding to the promoter region of G6PD. Therefore, the KHDRBS1/SNORD51/ZBED6 pathway performs an important part in regulating the pentose phosphate pathway to influence malignant biological behavior of GBM cells, providing new insights and potential targets for the treatment of GBM.
Materials and Methods (urval)
Cell migration assay
Cells were inoculated onto 6-well plates (Corning, NJ, USA) at appropriate amount and cultured under HoloMonitor M4 system for observation.
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| I The effect of KHDRBS1 knockdown on cell migration of U251 and U373 cells was analyzed by Hstudio M4 system. Scale bars: 100 µm. |
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| F The effect of SNORD51 on cell migration of U251 and U373 cells was analyzed by Hstudio M4 system. Scale bars: 100 µm. |
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| H The combined effects of KHDRBS1 and SNORD51 on cell migration of U251 and U373 cells was analyzed by Hstudio M4 system. Scale bars: 100 µm. |
Mvh the99
99:an, jag förstår inte riktigt, är inte holomonitorn färdig, kan bara användas pre kliniskt? Although the current HoloMonitor® (M4) is only applicable to the pre-clinical market segment, further optimization of the sales process is expected to yield improved outcomes. Kanske du kan förklara vad som saknas
SvaraRaderaHoloMonitorn är färdig ja,men för användning vid klinik tror jag det krävs certifiering av nåt slag. Inget PHI prioriterade initialt utan man tänkte väl att forskningsmiljön skulle vara tillräckligt stor för att få bra försäljning. Nu har det visat sig via Reg Med (behandling vid sängkanten) att det kliniska området är nåbart så man siktar även dit. Mvh the99
SvaraRaderaGmp och fda godkänd krävs.
SvaraRaderaDetta bolag hittar hela tiden nya saker att förändra och förbättra och tro att det skall resultera i något. Man hade intressenter 2019 utan allt detta. Hade de bara sålt tillräckligt många system för från början hade vi inte behövt vara här.
SvaraRaderaVem vet vad de hittar på härnäst, att M4an behöver för att ta "nästa steg"
Det togs fram en HoloMonitor 5 men man upptäckte att den inte behövdes för 4an kunde göra allt den kunde. Kommer inte ihåg när tyvärr.
SvaraRadera