HoloMonitor inlåst i Nature + Aussie research

Springer Nature koncernen har i en av sina utgåvor publicerad 28/4 en hårt inlåst studie innefattande användande av HoloMonitor. Mr google är dock på vår sida och kan bekräfta att så är fallet.

Lasso peptides: A focus on therapeutic index

Abstract

Lasso peptides, a significant group of ribosomally synthesized and post-translationally modified peptides (RiPPs), have emerged as potential therapeutic agents owing to their unique structural features and diverse biological activities. These peptides are characterized by an isopeptide-bonded macrolactam ring and a tail threaded through the ring, forming a distinctive lasso structure that provides remarkable stability and functionality, leading to significant therapeutic potential for antimicrobial, antiviral, anticancer, and anti-inflammatory applications. This review highlights the structural diversity and therapeutic applications of lasso peptides, emphasizing their potential as next-generation therapeutics. Additionally, while their mechanisms of action and innovative therapeutic prospects are explored, the challenges and limitations associated with their use are also discussed. Länk

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Har publicerat en studie om riktigt jobbig bröstcancer. Ett samarbetsprojekt mellan Australien,England och Quatar. 

A Short ERK5 Isoform Modulates Nucleocytoplasmic Shuttling of Active ERK5 and Associates with Poor Survival in Breast Cancer

Cell migration and motility assays

Real time cell motility assays were carried out using the HoloMonitor® M4 system (PHI, Lund Sweden) as per manufacturer instructions; 25-28 cells were tracked over 48 hours. Real time migration assays were performed using the xCELLigence system (CIM-Plate®; ACEA Biosciences Inc., San Diego, CA, USA). To promote cell migration, 20% serum and 100 nM estradiol (E2) were used as a chemoattractant in the base of the CIM-Plates.

Real time cell motility assays using the HoloMonitor® M4 system. Cell motility of 25-28 cells was tracked (left); inner and outer circles mark 50 and 100 μm distance, respectively. Comparison of cell motility over time (right) of the four MCF7 cell lines showed that only isoform-3 expressing cells had a significant increase (P<0.001, end-point comparison, one-way ANOVA). Cell migration assays, Transwell using CIM-plates, also showed increased cell migration of MCF7 cells expressing isoform-3.

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