Skrälldus med forskningsrapporter (20 st) +9

Här kommer en sammanfattning av senaste tidens publicerade forskningsrapporter. En del har tydliga hänvisningar till användande av HoloMonitor,andra är inlåsta men där Googles Scholar (GS) markerar användande.

Dual upregulation of miRNA-143 and miRNA-506 in non-small cell lung cancer inhibits proliferation, motility, migration, and tumor growth 06 June 2025

We performed the kinetic cell proliferation assay using the Holomonitor® Live Cell Imaging System (Phase Holographic Imaging, Sweden).

Dissecting Morphological and Functional Dynamics of Non-Tumorigenic and Triple-Negative Breast Cancer Cell Lines Using PCA and t-SNE Analysis 27 June 2025

The study employed a HoloMonitor M4, manufactured by Phase Holographic Imaging (PHI, Lund, Sweden). The Hstudio software package from PHI enabled time-lapse phase imaging, image manipulation, segmentation, and data examination. Images were captured at 15-min intervals, starting 24 h after cell seeding.

Exploring the pleiotropic effects of isoxazole derivative of usnic acid in breast cancer cells 14-08-2025

Cell migration (wound healing assay) was imaged on the Holomonitor (HM) for 72 hours.

NHE3 Controls Proliferation and Migration of Colonic Epithelial Cells 17 February 2025

… colonoids (HoloMonitor M4… HoloMonitor M4, PHI). Images were acquired every 20 min for
a total of 24 h, and the spontaneous migration across the gap was analyzed using HoloMonitor …

MENSTRUAL BLOOD-DERIVED MESENCHYMAL STROMAL CELL EXTRACELLULAR VESICLES STIMULATE STEM CELL AND CHONDROCYTE FUNCTIONS AND DIMINISH CARTILAGE DEGRADATION IN VITRO June 2025

GS

Dual-functional injectable hydrogels as antimicrobial and angiogenic therapeutics for dental pulp regeneration 

01 May 2025

Proliferation of endothelial cells was monitored using real-time live-cell imaging with the
HoloMonitor … Real-time wound coverage was monitored using the HoloMonitor system,

TNIK Regulates Cytoskeletal Organization to Promote Focal Adhesion Turnover and Mitosis in Lung Adenocarcinoma 22 May 2025

For the cell migration trajectory assay, A549 and PC-9 cells were seeded (2 $\times$ 105 cells/well) in a 6-well plate. After 12 h, the cells were monitored using HoloMonitor M4 (Phase Holographic Imaging PHI AB, Skiffervägen, Lund, Sweden) and photographs were taken every 5 minutes for 20 minutes. The cell migration trajectory was analyzed with HoloMonitor software (Version 2.7.1, Phase Holographic Imaging PHI AB, Skiffervägen, Lund, Sweden).

Utjecaj manoze i galaktoze na ekspresiju gena, kinetiku i proliferaciju mioblasta kod pacijenata s kongenitalnom mišićnom distrofijom 2025-08-26

3.5. Fazna holografska mikroskopija na mioblastima Nakon 24 sata pripremila sam HoloLid™ kako bih ga postavila na pločicu sa nasađenim mioblastima uz pomoć protokola proizvođača (Phase Holographic Imaging PHI, Švedska). Nakon što sam pažljivo provjerila da se u potpunosti osušio nakon sterilizacije, HoloLid™ sam postavila na pločicu s mioblastima, a pločicu smjestila na HoloMonitor® M4. Uređaj HoloMonitor® M4 koristila sam za neinvazivno praćenje stanica u stvarnom vremenu (Slika 7). Ovaj uređaj omogućuje holografsku mikroskopiju, odnosno 2D i 3D analizu mioblasta, uključujući promjene u obliku i površini stanica, njihovu diobu, migraciju i formiranje miotuba. HoloMonitor® M4 generirao je slike svakih 30 minuta na 10 različitih pozicija unutar svake jažice tijekom 72 h unutar CO2 inkubatora (5 % CO2, 37 °C). Nakon završetka snimanja, pločicu sa stanicama uklonila sam s uređaja i ostavila u CO2 inkubatoru (5 % CO2, 37 °C). 

Modified Nucleotides in Bifunctional Antisense Oligonucleotides: Exploring their Anticancer Potential 21 August 2025

For this purpose, we used holomonitor for real-time cell analysis that describes the influence
of modified BASOs on migratory properties of cancer cells by the distance that cells are able …

Unraveling Ammonia-Induced Brain Endothelial Senescence: Role of miRNA-183-5p 4 August 2025

First, we observed reduced by ∼20% RBE4 cell proliferation and captured by the live cell HoloMonitor recording of cell number. Results corroborate the observation from our laboratory, which demonstrated ammonia-induced RBE4 cell reduction with an MTT test assessing cell viability (Skowrońska et al., 2012). The cell parameters verified with HoloMonitor are presented in Supplementary Figure S1.

Stromal collagen IV expression and risk of breast cancer death in ductal carcinoma in situ 18 Jul, 2025

After seeding the cells were left to adhere for 4–6 hours, then washed with PBS. New DMEM was added, and the plates were inserted to the Holomonitor M4, which uses quantitative phase imaging to automatically detect and measure live cells without labelling.

The speed of individual cells between each consecutive captured image frame (distance travelled/time between frames) was calculated by the built-in software of the HoloMonitor, as well as the average speed of all cells of each type between consecutive frames. 

CLIC-Dependent Internalization of Caveolin-1 to Lysosomal Vacuoles in Response to Osmotic Regulation 20 Aug 2025

Imaging was performed with a HoloMonitor (PHI) quantitative phase imaging microscope …
Cells were segmented, tracked, and quantified at each timepoint using the HoloMonitor …

Multifunctional haemostatic and antibacterial wound dressing: Chitosan-silk fibroin composite with green-synthesised silver nanoparticles and deferoxamine 1 September 2025

GS

Relevance of transportome among the mechanisms of chemoresistance in hepatoblastoma July 2025

Cell viability was measured using MTT-formazan or sulforhodamine B assays, as previously reported, and proliferation rates were assessed via real-time digital holographic cytometry with a HoloMonitor® Live Cell Imaging System (Phase Holographic Imaging PHI AB, Lund, Sweden).

Spring festival firework activities exacerbate toxic effects of aerosol essential components 

15 June 2025

The real-time cell analysis and the Holomonitor (a quantitative phase contrast microscope) were used for investigated the toxic effects of aerosol on lung cells, and contaminants may show toxicity even at 1 mM. 

Intratumoral Heterogeneity of Vimentin Modulates Nuclear Mechanotransduction, DNA Damage Response and Cancer Cell Survival June 28, 2025

HoloMonitor

1×104 cells were plated on a12 well plate (Sarstedt) and holographic images were acquired for 7 days according to the HoloMonitor cell proliferation protocol. Five positions of two separate wells were acquired per condition. Automated quantification was obtained using the HoloMonitor App Suite.

Genetic inactivation of Alzheimer’s disease risk gene CD33 in human macrophages causes changes in gene signalling and cell function 

07 Apr 2025

Holomonitor and scanning electron microscopy were applied to examine changes in macrophage mobility and morphology following CD33 knockout.

Bioactive Phenolics from Vinegar-Egg Accelerates Acute Wound Healing by Activation of Focal Adhesion and Mitogen Activated Protein Kinase Signaling 2025 Aug 8

2.9. Time-Lapse Cell Tracking Analysis

For live cell migration tracking, MEF cells were seeded at a density of 9.0 × 10³ cells per 100 μL in DMEM lacking fetal bovine serum and supplemented with 2% Matrigel, using μ-Slide I chambers (Ibidi, #80106). After a 3 h pre-incubation period, 900 μL of DMEM containing 10% FBS was gently added to each well, followed by overnight incubation. Cells were subsequently treated with 100 μM of compounds 1–4 and immediately transferred to a HoloMonitor M4 imaging system (phase-holographic cytometry) maintained at 37 °C. Time-lapse images were acquired at 15 min intervals over a 12 h period, and cell motility was quantified using HoloStudio M4 analysis software (v2.6.4; Hstudio M4, Sun Valley, CA, USA).

Beyond Water Content: Unraveling Stiffness in Hydrated Materials by a Correlative Brillouin–Raman Approach June 21, 2025

4.3. Holographic Microscopy

Phase holographic images were acquired using a Holomonitor live cell imaging system (Phi). 

Jag har sparat den bästa studien till sist. 4 svenska forskare från Umeå Universitet har fått Nature att publicera deras forskning om bakterier kontra omkringliggande temperaturer i labbmiljöer.

3D-printed temperature and shear stress-controlled rocker platform for enhanced biofilm incubation Published 4 June 2025

To quantify biofilm thickness beyond the limitations of staining, we employed holographic imaging (HoloMonitor, Phase Holographic Imaging AB) on unstained replicates of Bt407 grown under identical conditions and seen in Fig. 6. This technique allowed us to assess biofilm coverage and thickness of up to 100 μm, which excluded some of the densest areas. 

Tillägg +9

Changes in Melanoma Cell Morphology Following Inhibition of Cell Invasion by Third-Generation mTOR Kinase Inhibitors 

Published: 12 August 2025 

Ligger nu även på amerikanska NIH

4.3. Quantitative Analysis of Cell Morphology and Motility

The In-depth Analysis: “Cell Morphology” module of the Holomonitor App Suite (PHI, Sweden) was used to track the movement of single cells. The following cell identification settings were used: automatic minimum error method, 134 adjustments, and minimum object size 20. Measurements of optical thickness, optical volume, roughness, shape convexity, irregularity, and motility were performed at specific time points. Using the “Kinetic Assays” module: “Kinetic Motility Assays”, In-depth Analysis: “Wound Healing Assay” was studied.

Transient CB2 receptor activation triggers irreversible luminal differentiation via chromatin remodeling in breast cancer Posted August 03, 2025.

To investigate cellular migration traits in detail, we employed the HoloMonitor® M4, an automated live-cell imaging cytometer that performs time-lapse analysis. This system allows for the imaging and quantification of unstained, living cells directly within their culture vessels inside the incubator, enabling real-time monitoring of various morphological and migratory parameters.

Cells prioritize the regulation of cell mass density 27 Aug 2025

En studie från John Hopkins University. Jag översätter texten om HoloMonitor då den är förklarande och bra.

Kvantitativ fasmikroskopi

För att mäta cellernas torrvikt använde vi Holomonitor M4 kvantitativt fasmikroskop (PHI). QPM bygger på principen att brytningsindexet för ett material omvänt korrelerar med hastigheten hos ljuset som passerar genom det. När Holomonitor är i drift riktar den en laserstråle genom både cellen och det omgivande odlingsmediet. När ljus passerar genom cellen, som har ett högre brytningsindex än mediet, saktar det ner, vilket resulterar i en detekterbar fasförskjutning. Denna fasförskjutning fångas av kameran och bearbetas för att sammanfatta den totala fasförändringen som orsakas av hela cellen (optisk volym). Den totala fasförändringen av enskilda celler analyserades med hjälp av den dedikerade programvaran Holomonitor M4. 

Development of targeted therapies against FET oncogene sarcomas 
2025-08-27 GUPEA

En Svensk doktorsavhandling från Sahlgrenska Academy måste naturligtvis uppmärksammas.

3.2.1 CELL VIABILITY ASSAYS AND HOLOMONITOR LIVE CELL MONITORING
To enable continuous monitoring of treatment response, a HoloMonitor live cell imaging system was used. This system employs laser-based quantitative phase imaging to assess cell number, confluence, and volume, without the need for chemical reagents (Figure 13). By eliminating reagent use, potential biases such as toxicity or uneven loading are avoided. Additionally, the system allows for real-time monitoring of cell lines prior to extraction and subsequent RNA and protein analyses. However, these experiments are more time consuming and require specialized holographic lids, as well as careful data interpretation and analysis to ensure reliable results. At the end of most HoloMonitor-based cell viability assays, alamarBlue was added to provide complementary data on metabolic viability. 

Ropivacaine and lidocaine at clinically relevant concentrations suppress proliferation and migration of ovarian cancer cells and induce morphological alterations  October 2025

2.6. Time-laps videos

Time lapse images were captured with a HoloMonitor® M4 digital holographic microscope with a motorized stage (M4) (PHI AB, Lund, Sweden) placed in a regular 37 ​°C CO2 incubator with 5 ​% CO2 in air. For time lapse-imaging of cells in the M4 microscope, cells were seeded in 6-well plates. The cells were allowed to attach for 24 ​h prior to start of the experiment. The standard lid of the Petri dish was replaced with a HoloLid® 71120 for 6-well plates (PHI AB). Cells were treated with 100 or 1000 ​μM lidocaine or ropivacaine. Control cells received PBS. The 6-well plate was then placed on the stage of the M4. The M4 is a quantitative imaging system based on digital holographic microscopy. When imaging with the M4 microscope, the software HoloMonitor® AppSuite was used. Images were acquired at 6–8 positions per well, with 5 ​min intervals for 72 ​h.

Caveolae and Rho Kinase: their implication of the senescent cell morphology and the secretion of the SASP in HeLa and A549 cancer cells

 Published: 17 September 2025

Studie från turkiska Gazi University. 

EFFECTS OF NIACINAMIDE ON KERATINOCYTE VIABILITY, PROLIFERATION, AND PROTECTION FROM OXIDATIVE STRESS 2025-06-02

Digital holographic microscopy (DHM) HaCaT cells were seeded into one 6-well plate from SARSTEDT with a cell concentration of 16’000 cells and left to adhere overnight before being replaced with EPILIFE medium and 1 and 10 mM NIA to get a final volume of 5 mL. Antibiotic with a concentration of 0.125 µg/mL amphotericin B from Thermo Fisher, Life Technologies, Gibco (Paisley, UK) was also added, the lid was replaced with HoloLids™ (Phase Holographic Imaging AB), and then placed on the DHM, HoloMonitor™ M4 (Phase Holographic Imaging AB, PHIAB, Lund, Sweden). The instrument was then calibrated for 10 minutes, incubated at 37°C with 5% CO2. Using the AppSuite™ software (Phase Holographic Imaging AB) of the instrument, 10 random positions were generated and left running for 72 hours in the same incubator in which it was calibrated, with images taken every two hours. Once the photos were taken, the software counted the number of cells and provided the cell count in x/cm2 and the confluency over time.

Plasmid-induced cytotoxicity revealed by nanopore and nanostraw electroporation - Nanoscale (RSC Publishing) 08 Sep 2025

2.7 Phase holographic microscopy

To study cell behavior on different types of nanosubstrates, cells were imaged using a phase holographic microscope (HoloMonitor, Phase Holographic Imaging PHI AB). The microscope and associated software (HoloMonitor App Suite) give 3D information about the cells without any perturbations since the measurements are performed in the incubator on unlabeled cells.

The Effect of Long-Term Betacoronavirus Infection on the Permeability of the Blood–Brain Barrier—In Vitro Model Studies 24 September 2025

3. Results

3.1. MHV-JHM Replication in bEnd.3 Cell Line

To identify a viral dose suitable for establishing long-term, non-cytolytic MHV-JHM infection in bEnd.3 cells, we monitored the proliferation of cultures infected with serial 10-fold dilutions of virus stock (100 to 10−5) over 72 h using HoloMonitor® PHI M4 (Phase Holographic Imaging, Lund, Sweden) live-cell imaging. Uninfected controls were included for comparison. Cell counts were recorded at 0 h and 72 h p.i., and statistical analyses were performed to assess both within-group changes and differences between conditions

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Det kan konstateras att de forskande HoloMonitoranvändarna världen över inte har legat på latsidan inför/under sommaren. Ovanstående är ett axplock som jag snabbt hittade.Den som vill ha in fler nya studier till den här listan kan mejla över dem så lägger jag till dem. 

                                            Mvh the99

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