Tjenixen
alla PHI,are.
Här kommer en tidig julklapp till oss alla aktieägare.
Idag (3 tim sen) publicerar tidskriften Nature en artikel som bla PHI,s främsta ambassadör, professor Stina Oredsson är medförfattare till.
www.nature.com/articles/srep18535
From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.
Published online:
22 December 2015
Artikeln, eller rättare sagt nya forskningsresultat, berättar om hur man med nanoteknik funnit nya tillämpingsområden inom cellbiologi.
Revolutionerande är kanske för starkt ord att använda, men uppseväckande ny info är det tydligen då tidskriften Nature väljer att ta in forskningsrapporten.
Stina m forskarkollegor har med PHI,s teknik lyckats ta fram ny information som kommer ha betydelse för framtida forskning kring hur celler och cellkärnor agerar resp interagerar vid specifika tillstånd och situationer.
Ur artikeln :
During recent years, vertical nanowire arrays have received increasing attention for their possible use in life sciences as electrodes biosensors as well as for axonal guidance, cell injections and anti-bacterial properties.
The rapidly expanding number of nanowire applications calls for a better understanding of the interactions between cells and nanowires, and, though steadily increasing, the number of papers studying cell-nanowire interactions remains low.
Some studies suggest that nanowires have little effect on cells, e.g. analyses of cellular mRNA content have shown no or limited changes in gene expression for cells cultured on nanowires compared to cells cultured on flat substrates. Similarly, cell functions such as protein expression and enzymatic activity have been shown to be unaffected by the presence of vertical nanowires.
The effects of nanowires on the cell membrane are not well understood either and seem to depend on cell type, nanowire density, interaction time span, and position of the nanowires with respect to the cell.
Using phase holographic microscopy, we captured 24–72?h long time-lapse movies of cells on the substrates (see Supplementary information, Movie S1-S5).
For each individual cell, the centre of mass was determined and followed over time.
The resulting track traces of the cells on the different substrates are shown in Fig. 2, visualizing the cell migration during the first 20?h after seeding.
The corresponding total displacement is summarized in Fig. 3.
Only the first 20 h were chosen in order not to bias the data toward slower moving cells, as faster cells can leave the field of view.
The cell motility is lower on all nanowire substrates compared to Polystyrene (PS) and GaP controls except on 4?µm-2 substrates, where cells move to a similar degree as those on PS and GaP.
For experiments involving phase holographic imaging (i.e. time-lapse and growth curves), we used substrates polished on both sides to enable imaging, as previously described.
The nanowire substrates were placed in 25?cm2 PS tissue culture flasks (one sample per flask) and the cells were suspended in warm medium and then added to the flasks in which the samples had been placed.
The flasks were then directly transferred to a phase holographic microscope (HoloMonitor M3, PHI AB, Lund, Sweden) kept in a 37?°C dry incubator where they were continuously imaged.
Images were saved every 5 min for 24 or 72?h.
The method yields 3D images with high contrast, which allows for cell identification and tracking on the sample using the phase holographic microscope software (HStudio 2.6, PHI AB, Lund, Sweden).
Detta är 2 gången PHI omnämns i ansedda Nature.
Mvh the99
Här kommer en tidig julklapp till oss alla aktieägare.
Idag (3 tim sen) publicerar tidskriften Nature en artikel som bla PHI,s främsta ambassadör, professor Stina Oredsson är medförfattare till.
www.nature.com/articles/srep18535
From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.
Published online:
22 December 2015
Artikeln, eller rättare sagt nya forskningsresultat, berättar om hur man med nanoteknik funnit nya tillämpingsområden inom cellbiologi.
Revolutionerande är kanske för starkt ord att använda, men uppseväckande ny info är det tydligen då tidskriften Nature väljer att ta in forskningsrapporten.
Stina m forskarkollegor har med PHI,s teknik lyckats ta fram ny information som kommer ha betydelse för framtida forskning kring hur celler och cellkärnor agerar resp interagerar vid specifika tillstånd och situationer.
Ur artikeln :
During recent years, vertical nanowire arrays have received increasing attention for their possible use in life sciences as electrodes biosensors as well as for axonal guidance, cell injections and anti-bacterial properties.
The rapidly expanding number of nanowire applications calls for a better understanding of the interactions between cells and nanowires, and, though steadily increasing, the number of papers studying cell-nanowire interactions remains low.
Some studies suggest that nanowires have little effect on cells, e.g. analyses of cellular mRNA content have shown no or limited changes in gene expression for cells cultured on nanowires compared to cells cultured on flat substrates. Similarly, cell functions such as protein expression and enzymatic activity have been shown to be unaffected by the presence of vertical nanowires.
The effects of nanowires on the cell membrane are not well understood either and seem to depend on cell type, nanowire density, interaction time span, and position of the nanowires with respect to the cell.
Using phase holographic microscopy, we captured 24–72?h long time-lapse movies of cells on the substrates (see Supplementary information, Movie S1-S5).
For each individual cell, the centre of mass was determined and followed over time.
The resulting track traces of the cells on the different substrates are shown in Fig. 2, visualizing the cell migration during the first 20?h after seeding.
The corresponding total displacement is summarized in Fig. 3.
Only the first 20 h were chosen in order not to bias the data toward slower moving cells, as faster cells can leave the field of view.
The cell motility is lower on all nanowire substrates compared to Polystyrene (PS) and GaP controls except on 4?µm-2 substrates, where cells move to a similar degree as those on PS and GaP.
For experiments involving phase holographic imaging (i.e. time-lapse and growth curves), we used substrates polished on both sides to enable imaging, as previously described.
The nanowire substrates were placed in 25?cm2 PS tissue culture flasks (one sample per flask) and the cells were suspended in warm medium and then added to the flasks in which the samples had been placed.
The flasks were then directly transferred to a phase holographic microscope (HoloMonitor M3, PHI AB, Lund, Sweden) kept in a 37?°C dry incubator where they were continuously imaged.
Images were saved every 5 min for 24 or 72?h.
The method yields 3D images with high contrast, which allows for cell identification and tracking on the sample using the phase holographic microscope software (HStudio 2.6, PHI AB, Lund, Sweden).
Detta är 2 gången PHI omnämns i ansedda Nature.
Mvh the99
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