Abstract 3455: Cystatin C decreases proliferation of melanoma cells by affecting mitosis length
Abstract
Cystatin
C is a small secreted protein, produced by most cells in the body and
found in significant quantities in all body fluids. It is the major
inhibitor of extracellular cysteine protease activity in mammals because
of its rapid binding to virtually all known cysteine proteases of
families C1 (cysteine cathepsins) and C13 (mammalian legumain).
Cystatin
C has also been shown to inhibit viral replication within cells and
display protective effects against cancer cell invasion.
The protein has
recently been shown to get internalized into cancer cells, providing an
explanation to possible intracellular effects of the inhibitor.
The
purpose of the present study was to elucidate possible direct effects
of cystatin C on cancer cell growth, by use of holometric imaging to
analyze proliferation rate as well as to monitor the mitosis phase in
real-time.
Human A375 melanoma cells were cultured with and without
addition of physiological quantities of cystatin C added to the culture
medium (1 micromolar final concentration).
The cultures were monitored
for up to 3 days in a standard incubator using a HoloMonitor M4
instrument.
Settings were optimized to allow accurate counting of cells
throughout the experiment by analysis of images captured, revealing an
inhibitory effect on overall cell proliferation rate by cystatin C
addition to the cultures.
To analyze the migration and cell division of
individual cells, images were captured at short intervals allowing
tracking and monitoring of cell morphology with respect to parameters
such as thickness/density and area.
Parameters could be set to define
start of the mitosis phase indicated by a distinct rounding-up of cells
and followed until a natural stop at cytokinesis, whereafter the
daughter cells could be continuously tracked.
These analyses revealed
qualitative differences in the normal migration behavior of the A375
cells under study as a result of cystatin C addition.
Moreover, the mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the actual mitosis phase.
Moreover, the mitosis phase was considerably prolonged in cells grown in cystatin C containing medium, indicating that the proliferation-inhibiting effect of the protease inhibitor may be through its direct action on proteolytic processes in the actual mitosis phase.
This finding
justifies in-depth studies on additional cancer cell types to reveal how
general the anti-proliferative effect of cystatin C is and how it can
be explored to possibly control cancer cell growth and spread.
The use
of real-time holometric imaging of growing cells should be a most useful
tool in such studies.
Citation Format:
Hanna Wallin, Kersti Alm, Magnus Abrahamson.
Cystatin C decreases
proliferation of melanoma cells by affecting mitosis length [abstract].
In: Proceedings of the American Association for Cancer Research Annual
Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR;
Cancer Res 2017;77(13 Suppl):Abstract nr 3455.
doi:10.1158/1538-7445.AM2017-3455
- ©2017 American Association for Cancer Research.
Min kommentar
Jag tror vi närmar oss det breda erkännandet av Digital Holografisk Mikroskopi som ett accepterat instrument att nå nya forskningsresultat.
Att sen PHI får äran att representera tekniken i en för cancerforskare känd tidskrift känns.....ja vad tycker ni alla PHI,are? Lite stolt å Bolagets vägnar tycker undertecknad man gott kan vara.
Tusen tack för ditt arbete The99, har läst dina poster/artiklar väldigt länge.
SvaraRaderaFramtiden ser minst sagt ljust ut för PHI. Detta kommer inte undgå dom stora drakarna i branchen.
Av 500 presentationer så valde dom ut dom där PHIs teknik användes. Det betyder att forskarna måste ha blivit väldigt imponerande..Tror vi kan se en högre efterfråga nu efter PHIs produkter.
Kan 2018 vara året som paradigm skiftet sker? Att PHI blir erkänt som den nya och optimala metoden för cytometri?
Det här är minst sagt spännande tider för PHI och Peter överdrev inte när han sa åt oss att tänka stort!
detta är minst sagt stort!!
tack igen för ditt grävande, mvh Cassus belli