Det är återigen kinesiska forskare i farten. Denna gång från Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery of Affiliated Hospital. En enhet vid Tianjin Medical University.
Rapporten
iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management
Abstract
This study aimed to investigate the effects of targeted temperature management (TTM) modulation on traumatic brain injury (TBI) and the involved mechanisms using quantitative proteomics technology.
SH-SY5Y and HT-22 cells were subjected to moderate stretch injury using the cell injury controller (CIC), followed by incubation at TTM (mild hypothermia, 32°C), or normothermia (37°C).
The real-time morphological changes, cell cycle phase distribution, death, and cell viability were evaluated.
Moderate TBI was produced by the controlled cortical impactor (CCI), and the effects of TTM on the neurological damage, neurodegeneration, cerebrovascular histopathology, and behavioral outcome were determined in vivo.
Results showed that TTM treatment prevented TBI-induced neuronal necrosis in the brain, achieved a substantial reduction in neuronal death both in vitro and in vivo, reduced cortical lesion volume and neuronal loss, attenuated cerebrovascular histopathological damage, brain edema, and improved behavioral outcome.
Using an iTRAQ proteomics approach, proteins that were significantly associated with TTM in experimental TBI were identified.
Importantly, changes in four candidate molecules (plasminogen [PLG], antithrombin III [AT III], fibrinogen gamma chain [FGG], transthyretin [TTR]) were verified using TBI rat brain tissues and TBI human cerebrospinal fluid (CSF) samples.
This study is one of the first to investigate the neuroprotective effects of TTM on the proteome of human and experimental models of TBI, providing an overall landscape of the TBI brain proteome and a scientific foundation for further assessment of candidate molecules associated with TTM for the promotion of reparative strategies post-TBI.
Rapporten är som vanligt låst, men vid sökning på den hittas den för PHI viktiga informationen:
The digital holographic microscope (HoloMonitor M4; Phase Holographic Imaging,
Lund, Sweden), placed in an incubator, was used to record 3-dimensional information
of injured cells after exposure to hypothermia or normothermia …
Artikeln publiceras i samarbete med
American Society for Experimental Neurotherapeutics
Och utgivare för denna tidskrift är Springer koncernen, SPRINGER NATURE.
Min kommentar
Det är lite retfullt att inte denna vetenskapliga artikel kom med i dagens delårsrapport.
I den nämner VD att det producerats 29 rapporter för 2017.
Med denna har vi alltså 30.
Artikeln ser ut att beröra ännu ett nytt forskningsområde vi inte stött på tidigare.
Reducera hjärnskada via temperaturreglering.
Förvisso nämner forskarna att deras studie är en av de första att undersöka effekten TTM, Targeted temperature management, har på skadan och dess omkringliggande område,vävnad.
Och PHI via deras HoloMonitor M4 är med på dessa nya insikter.
Wiki berättar :Targeted temperature management improves survival and brain function.....
Återigen ser vi alltså kinesiska forskare hitta nya forskningsområden där de med PHI,s eminenta teknik bidrar med nya insikter.
Inga kommentarer:
Skicka en kommentar