A Pseudomonas sp. DNE-S1 (GenBank accession number MF803832), able to degrade DEP in a wide range of acid-base conditions, was isolated from landfill soil. The growth kinetics of DNE-S1 on DEP followed the inhibition model. Fe3+ could promote the degradation ability of DNE-S1 to DEP probably by over-expression of the gene phthalate dihydrodiol dehydrogenase (ophB) and phthalate dioxygenase ferredoxin reductase (ophA4). The degradation rate of DEP (500 mg L−1 at 12 h) increased by 14.5% in the presence of Fe3+. Cu2+, Zn2+, and Mn2+
showed an inhibiting effect on the degradation performance of the
strain and could alter the cellular morphology, surface area and volume
of DNE-S1. Three degradation intermediates, namely ethyl methyl
phthalate (EMP), dimethyl phthalate (DMP), and phthalic acid (PA), were
detected in the biodegradation
of DEP, and the biochemical pathway of DEP degradation was proposed.
This study provides new information on the biochemical pathways and the
responsible genes involved in DEP degradation.
Studierna får även uppmärksamhet i kinesiska vetenskapliga kanaler. Hittas
här.
Måhända för att rapporten tar upp ett (för Kina) angeläget ämne. Nämligen miljöförstöring.
Som undertecknad förstår av abstractet handlar studien om hur effektivt bryta ner avfall som exempelvis innehåller kemikalier och metaller (tungmetaller?) med hjälp av viss typ av bakterier.
Att fokusera på dessa bakterier för att snabba på och mer effektivt få igång en nerbrytningsprocess.
Vi får ledord i artikeln som indikerar detta.
Pseudomonas
Pseudomonas
is a Gram-negative rod bacterial genus, which is ubiquitous in waters
and soils, being one of the most adaptable bacteria to various
environments.
Landfill
A landfill is an area of land that has been specifically engineered to allow for the deposition of waste onto and into it.
Phthalates
Phthalates
are synthetic chemical esters of phthalic acid that are widely used in a
variety of consumer products to impart flexibility, pliability and
elasticity to plastics and therefore known as “plasticizers”.
Dehydrogenases
Dehydrogenases
are intracellular enzymes that catalyze oxidation–reduction reactions
required for the respiration of organic compounds.
Biodegradation
Biodegradation
is the process by which organic substances are broken down into smaller
compounds by enzymes produced by living microbial organisms (in
biochemical processes, enzymes are organic substances that cause
chemical reactions without being consumed in the reaction.
Som belägg för att HoloMonitor använts i dessa studier ser vi att ett av dessa 3 institut finns upptagna på PHI´s lista över Users,
Northeast Agricultural University. De andra 2 finns däremot inte med på listan. Kanske de finns med i nästa uppdatering?
Ytterligare belägg hittas "bakvägen" när man söker på själva studien:
…
HoloMonitor M4 was used to analyze the effect of metal ions on the cell morphology,
including area, thickness, and volume. Before usage,
HoloMonitor M4 was calibrated,
with respect to exposure time, pattern contrast and hologram noise …
… All values were in the standard range, consisting of exposure time about 2–10 ms, pattern
contrast about 35–50 and hologram noise about 0.3–1.0. The images were analyzed
using the
HoloStudio 2.6.2. 2.6. RNA extraction and qPCR …
Med denna forskningsrapport kan vi konstatera att HoloMonitor använts inom ett helt nytt område.
Åtminstone för undertecknad är miljöförstöringsstudier inte bekant sen tidigare.
Jag tolkar det som att man använt HoloMonitor vid studier av "goda" bakterier.
Någon får rätta mig om jag har fel.
Avslutningsvis. Bloggen skrev tidigare "ketchupeffekt". Fortsätter informationsflödet som det gjort hittills får man nog kalla det för det.
Mvh the99
Tack för bra jobb med att hitta information. Denna var intressant.
SvaraRadera