Ny forskningsrapport från Norge

Tobias B.Beigl et al har fått sina studier granskade,godkända och publicerade nu i helgen (21/3).
Det handlar alltså om forskning kring den berömda Golgiapparatusen.Den känner ni igen väl? :-D
Hursom, forskarna har studerat hur det kroppsegna proteinet NAA80 i människas genuppsättning påverkar organellen Golgiapparatus vilken förekommer i eukaryota celler,alltså cell som återfinns i arvsmassan. 

Olika organeller i den eukaryota cellen.

Tobias m kollegor beskriver det enligt följande :

N-terminal acetylation of actin by NAA80 is essential for structural integrity of the golgi apparatus

Received 10 February 2020, Revised 11 March 2020, Accepted 15 March 2020, Available online 21 March 2020.

Abstract
N-alpha-acetyltransferase 80 (NAA80) was recently demonstrated to acetylate the N-terminus of actin, with NAA80 knockout cells showing actin cytoskeleton-related phenotypes, such as increased formation of membrane protrusions and accelerated migration. Here we report that NAA80 knockout cells additionally display fragmentation of the Golgi apparatus. We further employed rescue assays to demonstrate that this phenotype is connected to the ability of NAA80 to modify actin. Thus, re-expression of NAA80, which leads to re-establishment of actin's N-terminal acetyl group, rescued the Golgi fragmentation, whereas a catalytic dead NAA80 mutant could neither restore actin Nt-acetylation nor Golgi structure. The Golgi phenotype of NAA80 KO cells was shared by both migrating and non-migrating cells and live-cell imaging indicated increased Golgi dynamics in migrating NAA80 KO cells. Finally, we detected a drastic increase in the amount of F-actin in cells lacking NAA80, suggesting a causal relationship between this effect and the observed re-organization of Golgi structure. The findings further underscore the importance of actin Nt-acetylation and provide novel insight into its cellular roles, suggesting a mechanistic link between actin modification state and Golgi organization.

Jag klipper som vanligt in det PHI-relevanta.

Methods
For holographic imaging and analysis of live cells a HoloMonitor M4 (PHI AB, Sweden) was used. Data in form of holographic 3D time-lapse images were further processed in the HStudio software. 50,000 cells were seeded in 35 mm μ-dishes (ibidi) in 3 ml cell culture medium. 
This resulted in an initial cell confluency of 2–5%. Cells were imaged every 5 min with at least 3 different fields of view per dish.

Forskarna är snälla och bifogar 2 filmer i rapporten som visar användande av HoloMonitor.Jag lägger in den ena.Supplemental movie S3. Video from live-cell holographic imaging with HoloMonitor M4. HAP1 Ctrl cells. 3D holographic images acquired at 5 min interval for 13 hours (8 fps).
Kan ni inte se den får ni klicka på länken till forskningsrapporten och scrolla ner. 

Edit. Äras den som äras bör. Bloggen har ändrat uppgiften till att det är Tobias B. Beigl som står bakom huvuddelen i denna studie och inte kollegan Henriette Aksnes.