Ny forskningsrapport från Kina

För 18 timmar sen offentliggjordes en studie om cellomvandling som inte låter sig beskrivas närmare av en lekman som undertecknad.Ni får läsa den själva och lista ut kopplingen mellan cancerceller och deras omvandling.

Hursom,studien är inlämnad för granskning och kommer publiceras inom kort i ett adekvat vetenskapligt fora.

8 kinesiska forskare står bakom denna studie betitlad :

Self-organization of Tissue Growth by Interfacial Mechanical Interactions in Multi-layered Systems

Posted April 26, 2021. 

Abstract

Morphogenesis is a spatially and temporally regulated process involved in various physiological and pathological transformations. In addition to the associated biochemical factors, the physical regulation of morphogenesis has attracted increasing attention. However, the driving force of morphogenesis initiation remains elusive. Here, we show that during the growth of multi-layered tissues, morphogenetic process can be self-organized by the progression of compression gradient stemmed from the interfacial mechanical interactions between layers. In tissues with low fluidity, the compression gradient is progressively strengthened during growth and induces stratification by triggering symmetric-to-asymmetric cell division reorientation at the critical tissue size. In tissues with high fluidity, compression gradient is dynamic and induces cell junction remodelling regulated cell rearrangement leading to 2D in-plane morphogenesis instead of 3D deformation. Morphogenesis can be tuned by manipulating tissue fluidity, cell adhesion forces and mechanical properties to influence the progression of compression gradient during the development of cultured cell sheets and chicken embryos. Together, the dynamics of compression gradient arised from interfacial mechanical interaction provides a conserved mechanism underlying morphogenesis initiation and size control during tissue growth.

Under avsnittet Results hittar vi forskarnas användande av HoloMonitor.

                                                                           Figure 2.

 

 

(f) The representative live images of a growing HeLa cell sheet using HoloMonitor M4 time-lapse cytometer. Scale bar: 150 μm. (g) The statistical analysis of the area of individual cell during HeLa cell sheet growth.

                                                                           Figure 4.

(g) The representative images of dividing cells before (0 h) and after (75 h) critical compression during HeLa cell sheet growth using HoloMonitor M4 time-lapse cytometer.

Materials and Methods

Live imaging

Live cell imaging was performed in Leica microscope or HoloMonitor M4, enclosed in an incubator to maintain the samples at 37 °C and 5% of CO₂ throughout the experiments. Images were acquired every 10 min with Leica software. Spindle-rocking experiments were acquired every 3 min with Leica microscope. HoloMonitor M4 is a Quantitative phase imaging-based cell analyzer utilizing the principle of digital holographic microscopy. Live cell imaging was performed in HoloMonitor M4, enclosed in an incubator to maintain the samples at 37 °C and 5% of CO₂ throughout the experiments. Images were acquired every 10 min with HStudio 2.7.

Image processing, segmentation and quantification

Cell data analysis of HoloMonitor M4: Cell area, cell thickness, cell sheet area and cell sheet thickness were analyzed by the software HStudio 2.7. HStudio 2.7 can automatically segment and extract physical parameters of the cell. For more details, please refer to the official manual.

Supplementary Movies

Supplementary Movie 1. Symmetric cell division before critical compression during HeLa cell sheet growth visualized by HoloMonitor M4 time-lapse cytometer.

Supplementary Movie 2. Asymmetric cell division after critical compression during HeLa cell sheet growth visualized by HoloMonitor M4 time-lapse cytometer.

 

Min kommentar

Forskningen blir mer avancerad tycker jag.Denna studie är ett sådant exempel. Ta bara följande meningar och försök lista ut betydelse :

Morphogenesis is a common process that occurs widely in embryonic development, tissue regeneration and cancer progression.

Most biological tissues have multi-layered structures. The interactions between layers are essential in tissue homeostasis maintenance and morphogenesis during embryonic development and pathological progression.

For example, the interaction between cancer cells and their adjacent stroma plays a key role in the progression of the diseases, including tumour invasion.

Here, by combining in vitro and in vivo biological experiments, theoretical analysis and numerical simulations, we report a general mechanism underlying the initiation of morphogenesis driven by the progression of compression gradient stemmed from the interfacial mechanical interactions between growing tissue layers.

 

Och det som fick mig att ge upp försöken att förstå. Kyckling /fjädrar/skinn ??? 

 

Progressive compression gradient is strengthened in epidermal layer during chicken feather follicle morphogenesis

During the development of avian skin, the feather follicles develop by the stratification of single-layered epidermis.. In the in vitro culture of chicken skins, we found that the feather primordia emerged at Day 2 in cultured epidermis combined with dermis after isolation from HH30 stage embryos. However, when epidermal cell sheet was isolated and cultured alone (without dermal cell layer), it failed to stratify and no primordium was formed , indicating that the interaction between epidermal and dermal layers is essential for the morphogenetic process of epidermal cell sheet. Moreover, during the evolution of epidermis from monolayer to multilayer, the shapes of epidermal cells showed significant alteration from flat to columnar and correlated with the stages in embryo development and with the increased epidermal cell layer.

 

Hursom,vi ska vara tacksamma att forskarna bedriver studier på denna nivå.Kampen mot cancereländet måste fortsätta tills vi jämnat ut oddsen mot denna j--a best.

 

                                                             Mvh the99 

 

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