Live-Cell Imaging: A Balancing Act Between Speed, Sensitivity, and Resolution
First online: 30 November 2022
What You Will Learn in This Chapter
Live-cell imaging is perhaps one of the most exciting and challenging activities in the field of microscopy. It is exciting as recent developments in microscope technology have enabled scientists to visualize cellular and subcellular processes in real time down to the molecular level. With this comes the prospect of studying the mechanisms of diseases in greater detail and finding possible therapeutic solutions. Nevertheless, live-cell imaging is equally challenging because cells themselves and in fact—all cellular processes—are extremely sensitive to the very impact of using light for their visualization. The aim of this chapter is to provide a practical overview for early PhD students as well as more experienced post-docs, who will spend considerable time mastering the most important challenges and prerequisites in the very rapidly evolving field of live-cell microscopy.
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