7 forskningsrapporter + 1 (knorr)

Bloggen har haft ett glapp med att rapportera in nya forskningsrapporter,det mtp allt annat som hänt och händer i PHI. Men bara för det ligger forskarna inte på latsidan såklart. 😎  Här kommer ett gäng HoloMonitor baserade studier. Komprimerade för att alla ska få plats i 1 inlägg. Ni får klicka på resp rubrik och grotta ner er i de senaste rönen.

10 forskare från Taiwan har studerat temperatur som faktor vid framkallad celldöd,att få en skitcell att gå in i apoptos. Studien har utgått från 2 celltyper som initierar Alzheimers sjukdom och besten cancer. Ett nytt synsätt att ha ihjäl dessa skitceller innan de skapar elände för bäraren. Studien publiceras i Nature organet BMC,har även snappats upp av amerikanska NIH.

Dissociation of the nuclear WWOX/TRAF2 switch renders UV/cold shock-mediated nuclear bubbling cell death at low temperatures

Abstract

Background

Normal cells express functional tumor suppressor WW domain-containing oxidoreductase (WWOX), designated WWOXf. UV irradiation induces WWOXf cells to undergo bubbling cell death (BCD) — an event due to the accumulation of nuclear nitric oxide (NO) gas that forcefully pushes the nuclear and cell membranes to form one or two bubbles at room temperature (22 °C) and below. In contrast, when WWOX-deficient or -dysfunctional (WWOXd) cells are exposed to UV and/or cold shock, the cells undergo nuclear pop-out explosion death (POD). We aimed to determine the morphological and biochemical changes in WWOXf cells during BCD versus apoptosis.

Methods (urval)

WWOXf and WWOXd cells were exposed to UV followed by measuring BCD or POD by time-lapse microscopy and/or time-lapse holographic microscopy at 4, 22, or 37 °C to visualize morphological changes. Live cell stains were used to measure the kinetics of nitric oxide (NO) production and Ca2+ influx. Extent of cell death was measured by uptake of propidium iodide and by internucleosomal DNA fragmentation using agarose gel electrophoresis.

Materials and methods

Time-lapse bright field and fluorescent microscopy and time-lapse holography

Further, UV/cold shock-treated cells were imaged by time-lapse holographic microscopy (HoloMonitor M4, Phase Holographic Imaging PHI Inc., Boston, MA, USA) and analyzed by Hstudio M4 Tracking software.

Dramatic increases in cell thickness during apoptosis, but not BCD, determined by time-lapse holographic microscopy. A, B COS7 cells were exposed to UV and then cold shock at 4 °C for 12 h. Cell bubbles were observed, as determined by light and holographic microscopy, respectively.

Finally, by time-lapse holography, we have demonstrated for the first time that there is an increased thickness or height in dividing or apoptotic cells. Cell volume increase occurs normally in dividing cells. The tallness lasts less than 20 min, and then the cell divides. In contrast, during apoptosis, the increased cell height can last for 5 to 8 h, followed by a breakdown of the whole cell.

-----------------------------------------------------------------------------

Nästa forskningsrapport är även den signerad Taiwan. 11 forskare har studerat hur man kan få läkemedel mot cancer att bli effektivare till att även även angripa cancerceller som är på väg att metastasera.

Drug-Free Mesoporous Silica Nanoparticles Enable Suppression of Cancer Metastasis and Confer Survival Advantages to Mice with Tumor Xenografts

Abstract

Despite advancements in nanomedicine for drug delivery, many drug-loaded nanoparticles reduce tumor sizes but often fail to prevent metastasis. Mesoporous silica nanoparticles (MSNs) have attracted attention as promising nanocarriers. Here, we demonstrated that MSN-PEG/TA 25, with proper surface modifications, exhibited unique antimetastatic properties. In vivo studies showed that overall tumor metastasis decreased in 4T1 xenografts mice treated with MSN-PEG/TA 25 with a notable reduction in lung tumor metastasis. In vitro assays, including wound-healing, Boyden chamber, tube-formation, and real-time cell analyses, showed that MSN-PEG/TA 25 could modulate cell migration of 4T1 breast cancer cells and interrupt tube formation by human umbilical vein endothelial cells (HUVECs), key factors in suppressing cancer metastasis.

Materials and Methods (urval)

2.12. Three-Dimensional (3D) Live-Cell Holographic Imaging

HoloMonitor M4 holographic cytometry (Phase Holographic Imaging, Lund, Sweden) was housed in standard cell culture incubator conditions. Briefly, 4T1 cells were seeded at low confluency (105 cells/dish) onto 35 mm culture dishes in RPMI medium overnight and then coincubated with 200 μg/mL of MSN-PEG/TA 25 for another 24 h. Next, dishes were directly placed onto a HoloMonitor M4 holographic microscope, and time-lapse phase images were recorded at intervals of 15 min over 24 h. For individual cell tracking, the center of mass was determined, and the cell motility at each time point was further analyzed by HStudioM4 software (n = 5).

-----------------------------------------------------------------------

17 spanska forskare har studerat en riktigt eländig cancer som har sin början i levern och sen vandrar vidare i gallgångarna. Hur få denna cancer att bli mer mottaglig för befintliga cancerläkemedel,det då den är extremt svårbehandlad.

Sensitizing cholangiocarcinoma to chemotherapy by inhibition of the drug-export pump MRP3

Abstract

Aims

Drug export through ABC proteins hinders cancer response to chemotherapy. Here, we have evaluated the relevance of MRP3 (ABCC3) in cholangiocarcinoma (CCA) as a potential target to overcome drug resistance.

Materials and Methods (urval)

2.11. Proliferation assays

CCA (EGI-1) cells were cultured in 24-well plates (2 ×105 cells/well) coated with ibiTreat® polymer (Ibidi, Inycom, Madrid). After 24 h, the antitumor drug was added in the presence or absence of the potential inhibitor of MRP3 for a short (6 h) incubation time, after which the medium was replaced and cell proliferation was analyzed during 72 h using the HoloMonitor® Live Cell Imaging System (PHI, Boston, USA). If inhibitors were evaluated in combination with antitumor agents, cells were pre-incubated with the inhibitors for 2 h at 37°C before adding the combination, and the chemosensitizer was maintained until the end of the experiment.

------------------------------------------------------------------------

HoloMonitorfrälste Robert Horvath från Ungern medverkar i nedanstående studie där HoloMonitor gås igenom med ackuratess.

Label-free biomolecular and cellular methods in small molecule epigallocatechin-gallate research 

Commercially available digital holographic microscope HoloMonitor M4 (HM, PHI AB, Lund, Sweden) allows the imaging of live cells under physiological conditions in an incubator environment in real- and long periods. Each image captured contains data on more than 30 cellular parameters in every single cell. HM has essential applications in biology, providing quantitative information on living cells. It has been used to study the morphology and dynamics of biological cells, including the invasiveness of cancer cells, adhesion, proliferation, migration, and death.

Fig. 3 Holographic microscope method and the collection of the results.A. Working principle of the HM4: A laser beam is split into two identical beams (sample and reference), the sample beam transmitting through the study object and interfering with the reference beam creating a hologram. Based on ref [44]. B. Profile analysis of a single HeLa cell by HM4. 

Understanding diseases requires studying cell migration and motility, crucial factors in cancer progression. The effect of EGCG on cancer cell motility and migration was investigated using a digital holographic microscope HM4. The software of this system calculates motility as the distance the cell moves from start to end of the analysis and migration as the shortest distance between the two points. Adding EGCG (500 μg/mL) to HeLa cells resulted in decreased migration, as well as motility (Fig. 3C) and motility speed.

HM4 creates sharp 3D images of cells, but due to its limited vertical resolution, very thin parts of the cell lamellipodium may not be detected, leading to an underestimation of cell contact area and volume and an overestimation of cell thickness. A simple method has been proposed to correct this issue.

----------------------------------------------------------------------------

6 forskare från Litauen har gjort studier på stamceller där deras HoloMonitor använts. Tyvärr är studien hårt inlåst så ni får lita på undertecknad att det stämmer. Det är alltså PHI`s kund

• Lithuanian Center for Innovative Medicine

som står för innehållet i denna studie med mesenkymala celler.

CHONDROGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STROMAL CELLS IN CELL SHEETS UNDER PHYSIOXIA FOR CARTILAGE TISSUE REGENERATION

------------------------------------------------------------------------------

7 kinesiska forskare har återigen gett sig i kast med att försöka knäcka den fruktade hjärncancern glioblastoma.

Dual role of exosomal circCMTM3 derived from GSCs in impeding degradation and promoting phosphorylation of STAT5A to facilitate vasculogenic mimicry formation in glioblastoma

Abstract

Background: Glioblastoma (GBM) is characterized by abundant neovascularization as an essential hallmark. Vasculogenic mimicry (VM) is a predominant pattern of GBM neovascularization. However, the biological functions of circRNAs prompting VM formation in GBM remains unclarified.

Methods (urval)

Cell migration assay

Migration of DGCs was assessed by the HoloMonitor M4 culture system (Phase Holographic Imaging PHI AB, Lund, Sweden). Briefly, cells were seeded into a six-well plate at a density of 10⁴ cells/ml. After cultured and incubated with exosomes, cells were photographed by HoloMonitor M4 culture system every 2 h for a total of 12 h. Cell image of each group was recorded and stored at the last point in time, and then the cellular movement trajectory was simulated and analyzed.

G The migration ability of DGC01 is detected by HoloMonitor and visualized in Hstudio (n = 5) in different GDEs treatment groups. Scale bars = 50 μm.

----------------------------------------------------------------------

I en annan blastoma studie där cancern angriper bäraren i tidig ålder,barn och ungdomar, har 5 forskare från Spanien flitigt använt sig av HoloMonitor för att hitta bot mot denna amöba.

Real-time morphometric analysis of targeted therapy for neuroblastoma cell lines in monolayer and 3D hydrogels using digital holographic microscopy

Highlights

•

Digital Holography enables the assessment of neuroblastoma cell dynamics.

•

Vitronectin enhances cluster growth rate inside 3D hydrogels.

•

Cilengitide promotes cell aggregation in cell monolayers and 3D hydrogel borders.

•

Real-time monitoring of 3D models is a valuable tool for precision medicine.

---------------------------------------------------------------------------

I en nyligen avslutad konferens i Tjeckien där ämnet var mitokondrie-DNA vs celler medverkade Altium som sponsor och gjorde reklam för HoloMonitor.

First International Conference on Horizontal Mitochondrial Transfer and Dynamics

----------------------------------------------------------------------------

Avslutningsvis en hyperintressant studie från kompisarna i ReMDO, Axiom Space. Där svensken Marcus Wandt var med i Axiom Mission 3 som astronaut. I föregående rymdfärd Mission 2 har nu cellstudierna som bedrevs uppe i rymden granskats och publicerats i Nature.

Surface tension enables induced pluripotent stem cell culture in commercially available hardware during spaceflight

Introduction

Stem cell research on a variety of stem cell types in low Earth orbit (LEO) has revealed multiple opportunities for understanding fundamental changes to stem cell properties and potentially harnessing these changes for biomanufacturing applications in microgravity. Investigations into the effects of culturing stem cells in microgravity could identify novel mechanisms capable of improving biomanufacturing of cellular therapeutics on Earth, or serve as a catalyst for future large-scale, on-orbit biomanufacturing of stem cell-derived products.

Läs studien och bli impade över vad de upptäckt och som kommer ligga till grund för riktigt avancerad Reg Med celltillverkning där HoloMonitor kommer ha sin givna plats. Jag klistrar in en bild från kapseln som visar att astronauterna faktiskt satt och labbade friskt däruppe  i rymden. 

Et voilá ! Det händer massvis HoloMonitor/PHI relaterat kan konstateras.Fast aktien har fastnat i träsket är det full fart i forskningen. Mvh the99

Som service till alla HoloMonitornyfikna forskare : HoloMonitor Demo