HoloMonitor med i en omfattande studie

I en nyligen publicerad studie beträffande nanopartiklar och deras förmåga som "bärare" av cellgifter lyfter författarna fram PHI,s HoloMonitor som ett förträffligt instrument att använda vid observationer och uppföljning.
Det är i organet IntechOpen de polska forskarna Magdalena Jedrzejczak-Silicka and Ewa Mijowska fått sina studier granskade och nu publicerade.
Notera tidsfönstret från inlämning till godkännande och publicering.

Submitted: July 4th 2017 Reviewed: November 20th 2017 Published: July 25th 2018

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Studien heter

General Cytotoxicity and Its Application in Nanomaterial Analysis

och hittas här. 

Ur den citerar jag HoloMonitor relaterat. 

Övrig info med resultat och resonemang är för maffigt att beta sig igenom så jag nöjer mig med det som mest intresserar oss PHI,are.

 

- Holographic (transmission) microscopy is a high-resolution imaging technique that provides

label-free and non-invasive, non-phototoxic and non-destructive method for real-time live cell

culture analysis.

This type of microscopy allows for quantitative and qualitative measurements of living cells (not only cultures of mammalian cells, but also protozoan, bacterial and plant cells) and collecting information about cell surface area, cell viability and morphological changes, such as differentiation, proliferation, motility, cell death, confluence or cell segmentation (calculated from a particular hologram).

Traditional brightfield microscopy has some limitations, such as difficulties in visualising individual cells due to their low contrast properties, whereas DH microscopy provides possibility to determine cell number directly in cell culture vessels.

The size of the HoloMonitor™ M4 (Phase Holographic Imaging AB, Lund, Sweden)

makes it possible to place it in a cell culture incubator, so that cell observations can be conducted

over long periods of time without any changes in cell culture conditions.

Digital holographic microscopy also enables the formation of three-dimensional (3D) images of the observed objects.

Holographic phase imaging is an excellent tool for cell morphometric characterisation and cell migration studies. 

This technique has recently been applied in clinical diagnostics, e.g.,screening for malaria infection of erythrocytes, cancer cell analyses or sperm quality.

Interest in the use of DH microscopy in research is constantly increasing.

For example, Lajkó et al. analysed the effect of a drug based on GnRH-III (gonadotropin-releasing hormone-III) on melanoma cells.

Holographic phase imaging was used to visualise the migratory behaviour of melanoma cells in response to daunorubicin (Dau) coupled with GnRH-III and its derivatives (modified at position 4 with Lys(Ac) (conj1) or Lys(nBu) (conj2)).

Cell migration analysis showed increased migration activity when cells were exposed to conj1, whereas conj2 decreased melanoma cell activity and exerted an immobilising effect on tumour cell spreading; thus, it was a better candidate for targeted tumour therapy.

Monitoring of HeLa cancer cells and MC3T3-E1 preosteoblast cells via holographic technique was also conducted by Peter et al.

These authors evaluated cell movements and morphological parameters of cells in two experiments.

In the first one, the HoloMonitor™ M4 was used to detect the effect of EGCg (green tea—epigallocatechin gallate) on HeLa cell motility.

Time-lap images showed that migration, motility and the speed of motility were reduced after EGCg

was added to the culture. 

The second experiment involved MC3T3 plated on transparent titanate nanotubes (TNT) surface and the impact on adhesion and spreading process of the cells was demonstrated using the HoloMonitor.

The authors have concluded that holographic digital microscopy is a useful tool for cellular behaviour analysis, but some limitations have also been found.

Peter at al.observed that under certain thicknesses, some parts of the cells (e.g., parts of the thin lamellipodium) slicked into the background surface. It was caused by the limited vertical resolution of the optical system.

In our study, the effect of the h-BN-Au nanocomposite on L929 and MCF-7 cell lines was

analysed during 12-h incubation using the HoloMonitor™ M4.

L929 cells did not show any significant differences in the presence of the nanocomposite and the doubling time (DT) value was similar to DT obtained in the control culture.

The results obtained for the MCF-7 cell line incubated with h-BN-Au demonstrated a stronger effect on cells. The DT analysis using holographic technique indicated a high reduction of proliferation capacity (the DT value for the MCF-7 control sample was 25.95h, whereas for experimental cultures, it was 469.9 h).

Figure 6.

The morphology of the L929 and MCF-7 cell lines incubated with the h-BN-Au nanoparticles. L929 culture

time-points at 0h (A); at 12h (B); MCF-7 culture time-points at 0h (C); at 12h (D) [83].