I mejlkorgen

2 tips har kommit in till bloggen. Christian informerar att PHI nu finns representerade hos den Italienska återförsäljaren Twin Helix. Tämligen ny information av Linkedin att döma.

Såhär marknadsför man HoloMonitor (engelsk version)

PHI skickar hälsningar till vår nye representant i Italien,det via Linkedin.

Sen har Oscar varit ute med grävspaden och hittat nåt riktigt värdefullt.

För 1 år sen på dagen nästan köpte det EU-finansierade Centre for Innovative Medicine i Vilnus Litauen sin första HoloMonitor,det för att användas tillsammans med en inkubator från PHI`s partner BioSpherix.

Nu får vi resultat av deras forskning med HoloMonitor.Det inom området Regenerativ Medicin.

Ett första prov/bevis på HoloMonitors förträfflighet vid forskning inom Reg Med.

Denna forskningsrapport är mig veterligen den första inom Reg Med där HoloMonitor använts.

Förhoppningsvis kommer denna studie spridas brett inom forskarvärlden.

Kanske PHI kan använda den i sin marknadsföring riktad till den starkt framväxande sektorn Reg Med?

Studien hittas på sid 103 i ett kompendium från Vilnius universitet.Det finns även annan info om Reg Med-forskning om man orkar gå igenom hela kompendiumet.

DIFFERENT RESPONSE OF CHONDROCYTES AND MESENCHYMAL STEM CELLS TO BETAINE TREATMENT

Raminta Vaiciuleviciute1, Ilona Uzieliene1, Jolita Pachaleva1, Giedrius Kvederas2, Eiva

Bernotiene1

1Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine,

Vilnius, Lithuania

2Vilnius University, Faculty of Medicine, Vilnius, Lithuania

 

Osteoarthritis (OA) is a long-term joint disease and it is a leading cause of disability across the

world. Betaine is a modified amino acid acting as an osmoprotectant and a methyl-group donor with

hepatoprotective, neuroprotective and cardioprotective role. Its anti-inflammatory, antifibrotic and

antiangiogenic properties might lead to attenuation of osteoarthritic lesion progression in cartilage

and subchondral bone. However there is still limited knowledge about the effects of betaine on

chondrocytes and mesenchymal stem cells. The aim of our study was to compare the responses of

human chondrocytes and bone marrow mesenchymal stem cells (BMMSCs) to betaine treatment

under normal and inflammatory conditions.

Human chondrocytes were isolated from knee cartilage of OA patients undergoing joint

replacement and mesenchymal stem cells were derived from bone marrow. Cell proliferation was

measured by alamarBlue™, (fluorescence was measured at 560/590 nm), with/without betaine (10 mM)

pretreatment for 24 hours under normal and inflammatory (with IL-1β) conditions. Intracellular

calcium (iCa2+) levels were evaluated in both cell types as determined by the iCa2+ specific

fluorescent dye Cal-520, mean fluorescence was counted using ImageJ. 2 different doses of betaine

(10 mM and 40 mM) were used for morphological analysis by HoloMonitor® Live Cell Imaging System.

Betaine reduced proliferation of both chondrocytes and BMMSCs, but only in chondrocytes the

difference was significant. Under stimulation with with IL-1β, the proliferation of both cell types was

increased while the response to betaine pretreatment was different. Reduced proliferation of BMMSCs

and increased proliferation of chondrocytes was observed on the 5th day of IL-1β conditioning . ICa2+

analysis showed similar tendency in both cell groups as betaine increased iCa2+ level, but the

difference was significant only in BMMSCs group. Migration of BMMSCs was reduced by 10 mM betaine while upregulated by 40 mM betaine and comparing to control group, whereas no effects of betaine on chondrocyte migration were observed.

In concusion, betaine differently modulates responses in chondrocytes and BMMSCs and under

normal and inflammatory conditions. Although similar effects of betaine on proliferation and iCa2+

level of human chondrocytes and BMMSCs under normal conditions were observed, it differently

affected cell migration and modulated cell proliferation under inflammatory conditions.

Min kommentar

Av dessa 2 tips sticker naturligtvis den första Reg Med - HoloMonitor studien ut. Nu kan PHI visa svart på vitt hur lämplig deras teknik är för denna typ av forskning.Det bör ge eko i forskarvärlden.

Och med detta grävarbete slipper Oscar våndan av att behöva göra en sammanfattning av den egenframgrävda forskningsrapporten från GlycoImaging-gänget. 😎😎😎

Undertecknad kommer beta av den och göra en sammanställning att publiceras på bloggen inom kort.

Tack till Christian och Oscar för gediget grävjobb.

                                          Mvh the99